Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12438 | 5' | -54.9 | NC_003324.1 | + | 1516 | 0.7 | 0.549883 |
Target: 5'- gCCagCGAggaUCcggCGGCAGUGGUGCGCGGc -3' miRNA: 3'- aGGa-GCU---AGa--GCCGUUGCUACGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 5278 | 0.66 | 0.763391 |
Target: 5'- aUCCUCGAUCUCG-CuAUGAUcccgGC-CGGa -3' miRNA: 3'- -AGGAGCUAGAGCcGuUGCUA----CGcGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 6660 | 0.66 | 0.773399 |
Target: 5'- gCCUacCGAguUCUacgucuacccCGGCAACGAUGgGuCGGg -3' miRNA: 3'- aGGA--GCU--AGA----------GCCGUUGCUACgC-GCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 8746 | 0.74 | 0.317077 |
Target: 5'- aUCCa-GAUCUUGGCGGCGGUGU-CGGa -3' miRNA: 3'- -AGGagCUAGAGCCGUUGCUACGcGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 12481 | 0.66 | 0.772405 |
Target: 5'- aUCgUCGAUCUCGGUcACGcgGUcugcuacGCGu -3' miRNA: 3'- -AGgAGCUAGAGCCGuUGCuaCG-------CGCc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 13447 | 0.76 | 0.262634 |
Target: 5'- aUCCUCGAUCcguaCGGCAGCGcGUcgcauguuugguccgGCGCGGc -3' miRNA: 3'- -AGGAGCUAGa---GCCGUUGC-UA---------------CGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 15118 | 0.72 | 0.438282 |
Target: 5'- gCCaUCGGUCUCGGCGuCGAcacgGCGCc- -3' miRNA: 3'- aGG-AGCUAGAGCCGUuGCUa---CGCGcc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 16431 | 0.7 | 0.51835 |
Target: 5'- gUCCUggacgacgCGAUCgaCGGCAACGccGuCGCGGa -3' miRNA: 3'- -AGGA--------GCUAGa-GCCGUUGCuaC-GCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 17555 | 0.7 | 0.549883 |
Target: 5'- aCCaaaGGUC-CGGCAugcCGAUGCGCGa -3' miRNA: 3'- aGGag-CUAGaGCCGUu--GCUACGCGCc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 17710 | 0.71 | 0.45766 |
Target: 5'- aUCCUCGGgcccgagCUCGGCGgcaucgugcucaACGgcGCGcCGGg -3' miRNA: 3'- -AGGAGCUa------GAGCCGU------------UGCuaCGC-GCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 18406 | 0.67 | 0.694555 |
Target: 5'- aUCCUCGGcgcccgcuuuggcggUCUCaccaaggccggGGCAGCGAUugcccGCGCGu -3' miRNA: 3'- -AGGAGCU---------------AGAG-----------CCGUUGCUA-----CGCGCc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 18782 | 1.12 | 0.000893 |
Target: 5'- cUCCUCGAUCUCGGCAACGAUGCGCGGg -3' miRNA: 3'- -AGGAGCUAGAGCCGUUGCUACGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 19150 | 0.67 | 0.702032 |
Target: 5'- gUCCUUGAUCUUcuggagcgucugcuuGUAGCGGuccucUGCGCGGc -3' miRNA: 3'- -AGGAGCUAGAGc--------------CGUUGCU-----ACGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 20662 | 0.66 | 0.773399 |
Target: 5'- gCUUCGAcca-GGCGGCGAUGCuuGGa -3' miRNA: 3'- aGGAGCUagagCCGUUGCUACGcgCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 21323 | 0.67 | 0.74299 |
Target: 5'- aUCCUCGAUUUCGcGCGuuuucaGAuUGCccuuGCGGa -3' miRNA: 3'- -AGGAGCUAGAGC-CGUug----CU-ACG----CGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 22780 | 0.79 | 0.157811 |
Target: 5'- cCUUCGAcgagCUCGGCGugGAUGCGCu- -3' miRNA: 3'- aGGAGCUa---GAGCCGUugCUACGCGcc -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 24278 | 0.67 | 0.711598 |
Target: 5'- cUCgUCGAUCUgGGCAcggcggagcuugGCGAaggcGuCGCGGa -3' miRNA: 3'- -AGgAGCUAGAgCCGU------------UGCUa---C-GCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 24750 | 0.66 | 0.792979 |
Target: 5'- cCCUCGAgucgCUUGcGCAggauuACGAUGUcgGUGGu -3' miRNA: 3'- aGGAGCUa---GAGC-CGU-----UGCUACG--CGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 26482 | 0.7 | 0.560529 |
Target: 5'- -gCUCGAcCUCGGCAGCGcucgacauaccGUcgaauugcgugGCGCGGg -3' miRNA: 3'- agGAGCUaGAGCCGUUGC-----------UA-----------CGCGCC- -5' |
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12438 | 5' | -54.9 | NC_003324.1 | + | 26650 | 0.79 | 0.157811 |
Target: 5'- gUC-CGAUCUCGGCGAgGAUGUGCGu -3' miRNA: 3'- aGGaGCUAGAGCCGUUgCUACGCGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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