Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12445 | 3' | -56 | NC_003324.1 | + | 38349 | 0.66 | 0.674959 |
Target: 5'- -aGGGCAc-CGGGgcgGCGCUGguCGCCg -3' miRNA: 3'- agCCCGUacGCCUauaCGUGAC--GCGG- -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 56772 | 0.66 | 0.664041 |
Target: 5'- gCGGGCAggagGUGGAgccgGUAagcGUGCCg -3' miRNA: 3'- aGCCCGUa---CGCCUaua-CGUga-CGCGG- -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 42163 | 0.66 | 0.661853 |
Target: 5'- aUGGGCcgcgaucguacuUGCcGAacgacgGCACUGCGCCg -3' miRNA: 3'- aGCCCGu-----------ACGcCUaua---CGUGACGCGG- -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 42389 | 0.66 | 0.641028 |
Target: 5'- aUCGcGGUAUccuggucGCGGGUccGCAacaGCGCCa -3' miRNA: 3'- -AGC-CCGUA-------CGCCUAuaCGUga-CGCGG- -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 22065 | 0.66 | 0.664041 |
Target: 5'- gCGGGCuauggcgacuAUGcCGGcuccaagGUGCAUcGCGCCg -3' miRNA: 3'- aGCCCG----------UAC-GCCua-----UACGUGaCGCGG- -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 25518 | 0.66 | 0.664041 |
Target: 5'- gUGGGUcgGCuGGGgcgGC-CUGCGCa -3' miRNA: 3'- aGCCCGuaCG-CCUauaCGuGACGCGg -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 4662 | 0.66 | 0.684752 |
Target: 5'- cCGGGCcgacaucAUGCGGGccUGgAcCUGCGCg -3' miRNA: 3'- aGCCCG-------UACGCCUauACgU-GACGCGg -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 54937 | 0.66 | 0.664041 |
Target: 5'- cCGGGCcUGCGGGgucggaaccuUGaGgGCUGCGgCa -3' miRNA: 3'- aGCCCGuACGCCU----------AUaCgUGACGCgG- -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 55248 | 0.66 | 0.696665 |
Target: 5'- aCGuGCcgGCGGAcg-GCACcGCGCg -3' miRNA: 3'- aGCcCGuaCGCCUauaCGUGaCGCGg -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 157 | 0.66 | 0.685838 |
Target: 5'- uUCGGGauaGgGGAUAUuccuguagacgGCGC-GCGCCa -3' miRNA: 3'- -AGCCCguaCgCCUAUA-----------CGUGaCGCGG- -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 45828 | 0.66 | 0.684752 |
Target: 5'- -gGGGC-UGCGGcgagaacagugagGUGgaaacgGCGCgggGCGCCu -3' miRNA: 3'- agCCCGuACGCC-------------UAUa-----CGUGa--CGCGG- -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 29876 | 0.66 | 0.674959 |
Target: 5'- aUCaGGCAggGUGGAUAcgGCGCgaucuucgGCGCg -3' miRNA: 3'- -AGcCCGUa-CGCCUAUa-CGUGa-------CGCGg -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 24005 | 0.66 | 0.664041 |
Target: 5'- -aGGGCG-GCGGuaggGCuGCUGCGUg -3' miRNA: 3'- agCCCGUaCGCCuauaCG-UGACGCGg -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 6333 | 0.67 | 0.620174 |
Target: 5'- cUUGGGCAUGUcuuaccucuGGcgcuUGCugcgcugcuGCUGCGCCu -3' miRNA: 3'- -AGCCCGUACG---------CCuau-ACG---------UGACGCGG- -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 30748 | 0.67 | 0.598264 |
Target: 5'- cUCGGGaGUGaCGGAagcacggGCGCgguccUGCGCCg -3' miRNA: 3'- -AGCCCgUAC-GCCUaua----CGUG-----ACGCGG- -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 26790 | 0.67 | 0.609209 |
Target: 5'- uUCGgcuuGGCAgacGCGGccGUGCGCUuggcuggcuuugGCGCCg -3' miRNA: 3'- -AGC----CCGUa--CGCCuaUACGUGA------------CGCGG- -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 35538 | 0.67 | 0.63115 |
Target: 5'- gUUGGuggcugugaucuGCGaGCGGAUcUGCAaggcCUGCGCCu -3' miRNA: 3'- -AGCC------------CGUaCGCCUAuACGU----GACGCGG- -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 25196 | 0.68 | 0.565634 |
Target: 5'- cCaGGCAUGagaccaaGGAaguUGC-CUGCGCCg -3' miRNA: 3'- aGcCCGUACg------CCUau-ACGuGACGCGG- -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 26180 | 0.68 | 0.575382 |
Target: 5'- gUCGGcggucucGC-UGCGGGgcUGCGuCUGCGUCg -3' miRNA: 3'- -AGCC-------CGuACGCCUauACGU-GACGCGG- -5' |
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12445 | 3' | -56 | NC_003324.1 | + | 55033 | 0.68 | 0.576468 |
Target: 5'- cCGGGCAggcaagGCGGAcggcUGgGCUGcCGCg -3' miRNA: 3'- aGCCCGUa-----CGCCUau--ACgUGAC-GCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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