Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12452 | 5' | -50.7 | NC_003324.1 | + | 25010 | 0.68 | 0.851659 |
Target: 5'- -cGAucGAGACAcugcGACCGggcGGCGuGCUGGCg -3' miRNA: 3'- ccCU--UUCUGU----CUGGUa--UUGC-CGACCG- -5' |
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12452 | 5' | -50.7 | NC_003324.1 | + | 6275 | 0.7 | 0.764882 |
Target: 5'- aGGcGAGcgAGAUCAU-GCGGCUGGCc -3' miRNA: 3'- cCCuUUCugUCUGGUAuUGCCGACCG- -5' |
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12452 | 5' | -50.7 | NC_003324.1 | + | 45845 | 0.7 | 0.764882 |
Target: 5'- cGGAAGGcgugGCAGGCCAUGAuCGcuccGCUGGa -3' miRNA: 3'- cCCUUUC----UGUCUGGUAUU-GC----CGACCg -5' |
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12452 | 5' | -50.7 | NC_003324.1 | + | 18994 | 0.72 | 0.666548 |
Target: 5'- cGGGAAAuacGGCGG-CUGcacGCGGCUGGCc -3' miRNA: 3'- -CCCUUU---CUGUCuGGUau-UGCCGACCG- -5' |
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12452 | 5' | -50.7 | NC_003324.1 | + | 4683 | 0.72 | 0.6643 |
Target: 5'- aGGAAcauGACcugcgcaacaacGACCAgGGCGGCUGGCu -3' miRNA: 3'- cCCUUu--CUGu-----------CUGGUaUUGCCGACCG- -5' |
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12452 | 5' | -50.7 | NC_003324.1 | + | 37987 | 0.72 | 0.644016 |
Target: 5'- gGGGuuAGGCGG-CCAUGccggagcgguuGCGGCgcacggGGCg -3' miRNA: 3'- -CCCuuUCUGUCuGGUAU-----------UGCCGa-----CCG- -5' |
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12452 | 5' | -50.7 | NC_003324.1 | + | 7813 | 0.73 | 0.598898 |
Target: 5'- cGGAcauAGGCAGGCCAUG-CGGCcGuGCu -3' miRNA: 3'- cCCUu--UCUGUCUGGUAUuGCCGaC-CG- -5' |
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12452 | 5' | -50.7 | NC_003324.1 | + | 26783 | 0.77 | 0.399433 |
Target: 5'- cGGGAGcuucggcuuggcAGACGcGGCCGUGcgcuUGGCUGGCu -3' miRNA: 3'- -CCCUU------------UCUGU-CUGGUAUu---GCCGACCG- -5' |
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12452 | 5' | -50.7 | NC_003324.1 | + | 31596 | 1.16 | 0.001067 |
Target: 5'- cGGGAAAGACAGACCAUAACGGCUGGCg -3' miRNA: 3'- -CCCUUUCUGUCUGGUAUUGCCGACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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