Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12454 | 3' | -64.7 | NC_003324.1 | + | 41818 | 0.66 | 0.286836 |
Target: 5'- gUUGGGGGUGCCGugGCgUGaucGCa -3' miRNA: 3'- uGACCCCCGCGGUugCGgACggcCGg -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 46742 | 0.66 | 0.280107 |
Target: 5'- aGCacGGGGCGCCGAgGUgUGCCccgcGCUa -3' miRNA: 3'- -UGacCCCCGCGGUUgCGgACGGc---CGG- -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 32449 | 0.67 | 0.254443 |
Target: 5'- cGCUGGcaacGGCaGCCAugcccucguCGCCUucGCCGGCa -3' miRNA: 3'- -UGACCc---CCG-CGGUu--------GCGGA--CGGCCGg -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 7631 | 0.67 | 0.254443 |
Target: 5'- --cGGaGGCGU--GCGCCuUGCUGGCCc -3' miRNA: 3'- ugaCCcCCGCGguUGCGG-ACGGCCGG- -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 48724 | 0.67 | 0.247732 |
Target: 5'- --aGGGcgccaaccgcgucGGCGCCAGCaGgCUGCaGGCCa -3' miRNA: 3'- ugaCCC-------------CCGCGGUUG-CgGACGgCCGG- -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 30310 | 0.67 | 0.242351 |
Target: 5'- --cGGuGGCGUCGcucgGCGCgUGCUGGCUg -3' miRNA: 3'- ugaCCcCCGCGGU----UGCGgACGGCCGG- -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 14570 | 0.67 | 0.242351 |
Target: 5'- -aUGGGucgcGGCcuGCCGGCGCaagcgGCCGGCg -3' miRNA: 3'- ugACCC----CCG--CGGUUGCGga---CGGCCGg -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 44423 | 0.67 | 0.236487 |
Target: 5'- uGCgGGcaccGGGCGCCGAgacuucgguCGCUgaugGCCGGCg -3' miRNA: 3'- -UGaCC----CCCGCGGUU---------GCGGa---CGGCCGg -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 14696 | 0.67 | 0.236487 |
Target: 5'- --aGGGccguagcuccGGcCGCCAcgcuaccgccuGCGCC-GCCGGCCg -3' miRNA: 3'- ugaCCC----------CC-GCGGU-----------UGCGGaCGGCCGG- -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 14423 | 0.67 | 0.230174 |
Target: 5'- -aUGGGGaucaCGCCcggcucuGGCGCCcaUGCCGGCg -3' miRNA: 3'- ugACCCCc---GCGG-------UUGCGG--ACGGCCGg -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 38919 | 0.67 | 0.224558 |
Target: 5'- aGCUGGuGGGaCGCCGACGaauacgccauggaCCcGCaCGGCg -3' miRNA: 3'- -UGACC-CCC-GCGGUUGC-------------GGaCG-GCCGg -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 19896 | 0.68 | 0.220151 |
Target: 5'- cCUGGGacacauugcagagcuGGCGCCAACaCCUGUCGuucguccagaaaauGCCg -3' miRNA: 3'- uGACCC---------------CCGCGGUUGcGGACGGC--------------CGG- -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 16251 | 0.68 | 0.219605 |
Target: 5'- aGCUGcGGugcccgccucGGUGCCAGCgauGCCUgGCgCGGCCc -3' miRNA: 3'- -UGAC-CC----------CCGCGGUUG---CGGA-CG-GCCGG- -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 10677 | 0.68 | 0.20893 |
Target: 5'- gACUGGGGGacgguuGCCGuUGCCUGCauccGCUg -3' miRNA: 3'- -UGACCCCCg-----CGGUuGCGGACGgc--CGG- -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 41356 | 0.68 | 0.203762 |
Target: 5'- cGCUGGGGGCgGCuUAAUGaaguaGCCGGCa -3' miRNA: 3'- -UGACCCCCG-CG-GUUGCgga--CGGCCGg -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 16034 | 0.68 | 0.203762 |
Target: 5'- gACUGGcuGGUGaugaggccaaguUCGACGaCUUGCCGGCCg -3' miRNA: 3'- -UGACCc-CCGC------------GGUUGC-GGACGGCCGG- -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 51396 | 0.68 | 0.202234 |
Target: 5'- --cGGGcGGauaGUCuuccguaugaucguGACGCCUGCCGGCg -3' miRNA: 3'- ugaCCC-CCg--CGG--------------UUGCGGACGGCCGg -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 35017 | 0.69 | 0.183254 |
Target: 5'- --aGGGcGGCGCagaugaagagcaggAGCGCCcggcaacguggauccUGCCGGCCg -3' miRNA: 3'- ugaCCC-CCGCGg-------------UUGCGG---------------ACGGCCGG- -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 26015 | 0.69 | 0.179561 |
Target: 5'- --cGGGGugacugagcGCGCCAAUggcgucaccgGUCUGCCGGCg -3' miRNA: 3'- ugaCCCC---------CGCGGUUG----------CGGACGGCCGg -5' |
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12454 | 3' | -64.7 | NC_003324.1 | + | 29880 | 0.69 | 0.165867 |
Target: 5'- gGCaGGGuggauacGGCGCgaucuuCGGCGCgCUGCUGGCCa -3' miRNA: 3'- -UGaCCC-------CCGCG------GUUGCG-GACGGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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