miRNA: 3'- -AGCgGaaGCGGCCGUCGG-------------AGCCGCua -5'
miRNA: 3'- aGCGGAAgCGGCCGuCGGaGCCGCua -5'
miRNA: 3'- aGCGGAa--------GCGGCCGUCgGaGCCGCUa -5'
miRNA: 3'- agCGGAag--CGGCcGUCGGaGCCGCUa -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12456 | 5' | -62.4 | NC_003324.1 | + | 16996 | 0.66 | 0.371479 |
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Target: 5'- aUCGgUgaCGCUGGCAGCCgccaccuugcaaaUCGGCGc- -3' miRNA: 3'- -AGCgGaaGCGGCCGUCGG-------------AGCCGCua -5' |
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| 12456 | 5' | -62.4 | NC_003324.1 | + | 21076 | 0.66 | 0.372318 |
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Target: 5'- cUGCCUU-GUCGGCcGCCgCGGCGc- -3' miRNA: 3'- aGCGGAAgCGGCCGuCGGaGCCGCua -5' |
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| 12456 | 5' | -62.4 | NC_003324.1 | + | 26129 | 0.66 | 0.372318 |
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Target: 5'- gCGCUUgagcuagagCGCCGGCAGaC-CGGUGAc -3' miRNA: 3'- aGCGGAa--------GCGGCCGUCgGaGCCGCUa -5' |
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| 12456 | 5' | -62.4 | NC_003324.1 | + | 56135 | 0.66 | 0.372318 |
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Target: 5'- -aGCCUggaaGCCGaCGGCCgUGGCGAc -3' miRNA: 3'- agCGGAag--CGGCcGUCGGaGCCGCUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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