Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12457 | 5' | -55.7 | NC_003324.1 | + | 7427 | 0.74 | 0.277974 |
Target: 5'- uGAGuaccaACCGCAucgucagcgugccCUCGgcGCGCGGAACGACg -3' miRNA: 3'- -CUC-----UGGCGU-------------GAGUa-CGCGCCUUGCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 28552 | 0.7 | 0.438054 |
Target: 5'- aAGGCCaCGCUCGUGaCGUGGAACa-- -3' miRNA: 3'- cUCUGGcGUGAGUAC-GCGCCUUGcug -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 27184 | 0.7 | 0.474671 |
Target: 5'- -cGACCGCGCUgGUGUGCugcgccagaugcagGGAGCcGCa -3' miRNA: 3'- cuCUGGCGUGAgUACGCG--------------CCUUGcUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 18961 | 0.69 | 0.507532 |
Target: 5'- -cGACCGaGC-CGUGCgugaucuGCGGGACGACu -3' miRNA: 3'- cuCUGGCgUGaGUACG-------CGCCUUGCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 8678 | 0.69 | 0.508575 |
Target: 5'- cGAaACCGCGCcCGUGaCGCGGuccuCGGCa -3' miRNA: 3'- -CUcUGGCGUGaGUAC-GCGCCuu--GCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 14079 | 0.68 | 0.572568 |
Target: 5'- uGAGGUCaCACUCAUGCGCG--ACGGCc -3' miRNA: 3'- -CUCUGGcGUGAGUACGCGCcuUGCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 22095 | 0.67 | 0.605319 |
Target: 5'- aAGGCUGCGCUCGacaUGCgccucgucgauGCGGGcuaugGCGACu -3' miRNA: 3'- cUCUGGCGUGAGU---ACG-----------CGCCU-----UGCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 26911 | 0.67 | 0.627282 |
Target: 5'- -cGACgagGCACUCA---GCGGAGCGGCg -3' miRNA: 3'- cuCUGg--CGUGAGUacgCGCCUUGCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 33453 | 0.67 | 0.649262 |
Target: 5'- -uGGCCGgAgUUgcGCGCGGAGcCGGCa -3' miRNA: 3'- cuCUGGCgUgAGuaCGCGCCUU-GCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 6699 | 0.66 | 0.660233 |
Target: 5'- -uGACCugacGCACUUccuUGCGGAACGGCa -3' miRNA: 3'- cuCUGG----CGUGAGuacGCGCCUUGCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 51434 | 0.66 | 0.660233 |
Target: 5'- -cGugCGCGCUCGaaUGCaUGGAAgGGCu -3' miRNA: 3'- cuCugGCGUGAGU--ACGcGCCUUgCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 35483 | 0.66 | 0.660233 |
Target: 5'- cGAGGgCGaggaaAC-CAUGCGCGG-GCGGCc -3' miRNA: 3'- -CUCUgGCg----UGaGUACGCGCCuUGCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 31628 | 0.66 | 0.660233 |
Target: 5'- cGAGACCGCggGCgcgCGUG-GCGGccauucucACGACg -3' miRNA: 3'- -CUCUGGCG--UGa--GUACgCGCCu-------UGCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 1529 | 0.66 | 0.714485 |
Target: 5'- -cGGCgGCAgUgGUGCGCGGcgcuAUGGCc -3' miRNA: 3'- cuCUGgCGUgAgUACGCGCCu---UGCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 32683 | 1.1 | 0.000758 |
Target: 5'- aGAGACCGCACUCAUGCGCGGAACGACa -3' miRNA: 3'- -CUCUGGCGUGAGUACGCGCCUUGCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 39331 | 0.66 | 0.671179 |
Target: 5'- cGAGaaGCCGCGCUg--GCGagccuuCGGAACGAUg -3' miRNA: 3'- -CUC--UGGCGUGAguaCGC------GCCUUGCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 20465 | 0.67 | 0.638274 |
Target: 5'- uGAGAaCGCGCUCGaucgccgcauCGCGGAGCGGg -3' miRNA: 3'- -CUCUgGCGUGAGUac--------GCGCCUUGCUg -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 367 | 0.67 | 0.638274 |
Target: 5'- cGGACagGguCUCA-GCGCGGcGCGGCu -3' miRNA: 3'- cUCUGg-CguGAGUaCGCGCCuUGCUG- -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 6544 | 0.67 | 0.605319 |
Target: 5'- cGAGcuauCCGCACUUGUGCaCGGGAUGu- -3' miRNA: 3'- -CUCu---GGCGUGAGUACGcGCCUUGCug -5' |
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12457 | 5' | -55.7 | NC_003324.1 | + | 3425 | 0.68 | 0.583447 |
Target: 5'- -uGGCUGCACUUccGCGUGGGAaaGCa -3' miRNA: 3'- cuCUGGCGUGAGuaCGCGCCUUgcUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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