Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12458 | 5' | -49.7 | NC_003324.1 | + | 42519 | 0.66 | 0.970628 |
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Target: 5'- gCCGAU---UCGGUGACGGguGCUCGc -3' miRNA: 3'- -GGUUAggaAGCCACUGCUacUGAGCu -5' |
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| 12458 | 5' | -49.7 | NC_003324.1 | + | 12659 | 0.68 | 0.925684 |
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Target: 5'- aCAAcCUUUCGGcgucUGGCGAacgcaucgcUGGCUCGAu -3' miRNA: 3'- gGUUaGGAAGCC----ACUGCU---------ACUGAGCU- -5' |
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| 12458 | 5' | -49.7 | NC_003324.1 | + | 30563 | 0.68 | 0.913311 |
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Target: 5'- uCCGAgggCCUgcUCGGcGGCGccGAUUCGAc -3' miRNA: 3'- -GGUUa--GGA--AGCCaCUGCuaCUGAGCU- -5' |
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| 12458 | 5' | -49.7 | NC_003324.1 | + | 49242 | 0.71 | 0.806835 |
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Target: 5'- gUCGAUggugCCUUCGGUGGCGAcguUGAUcaccucaaUCGAg -3' miRNA: 3'- -GGUUA----GGAAGCCACUGCU---ACUG--------AGCU- -5' |
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| 12458 | 5' | -49.7 | NC_003324.1 | + | 4192 | 0.72 | 0.756198 |
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Target: 5'- gCCGA-CCUaCGGcGACGAgcGACUCGAg -3' miRNA: 3'- -GGUUaGGAaGCCaCUGCUa-CUGAGCU- -5' |
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| 12458 | 5' | -49.7 | NC_003324.1 | + | 38799 | 0.72 | 0.756198 |
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Target: 5'- -uGGUCCUUCGGcucGAUGAUGACgccgugCGGg -3' miRNA: 3'- ggUUAGGAAGCCa--CUGCUACUGa-----GCU- -5' |
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| 12458 | 5' | -49.7 | NC_003324.1 | + | 22952 | 0.72 | 0.745627 |
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Target: 5'- uCCAGUCUc-CGGUGGCGAUG-CgagCGAa -3' miRNA: 3'- -GGUUAGGaaGCCACUGCUACuGa--GCU- -5' |
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| 12458 | 5' | -49.7 | NC_003324.1 | + | 17004 | 0.73 | 0.668955 |
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Target: 5'- aCCGAUCUaUCGGUGACGcUGGCa--- -3' miRNA: 3'- -GGUUAGGaAGCCACUGCuACUGagcu -5' |
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| 12458 | 5' | -49.7 | NC_003324.1 | + | 33710 | 1.12 | 0.003138 |
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Target: 5'- uCCAAUCCUUCGGUGACGAUGACUCGAg -3' miRNA: 3'- -GGUUAGGAAGCCACUGCUACUGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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