Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12459 | 3' | -50.6 | NC_003324.1 | + | 14464 | 0.66 | 0.922975 |
Target: 5'- ----cUCGGUUGCGGCgcguugauugGCAgCaGCCg -3' miRNA: 3'- gauauAGCUAACGCCGaa--------CGU-GcCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 30460 | 0.66 | 0.928929 |
Target: 5'- ---gGUCGAggGCGGC----AUGGCCg -3' miRNA: 3'- gauaUAGCUaaCGCCGaacgUGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 47093 | 0.66 | 0.939952 |
Target: 5'- -----cCGAcacgUGCGGCgagGUGCGGCa -3' miRNA: 3'- gauauaGCUa---ACGCCGaa-CGUGCCGg -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 27909 | 0.66 | 0.934587 |
Target: 5'- ---cGUCGAa-GCGGCcacgUGCGgGGUCa -3' miRNA: 3'- gauaUAGCUaaCGCCGa---ACGUgCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 22021 | 0.66 | 0.928929 |
Target: 5'- -cAUGUCGAgcGCaGcCUUGCGCGcGUCc -3' miRNA: 3'- gaUAUAGCUaaCGcC-GAACGUGC-CGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 1520 | 0.66 | 0.928929 |
Target: 5'- -----gCGAggauccgGCGGCaguggUGCGCGGCg -3' miRNA: 3'- gauauaGCUaa-----CGCCGa----ACGUGCCGg -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 14569 | 0.66 | 0.922975 |
Target: 5'- ---cAUgGGUcGCGGCcUGCcggcgcaaGCGGCCg -3' miRNA: 3'- gauaUAgCUAaCGCCGaACG--------UGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 6786 | 0.66 | 0.922975 |
Target: 5'- ---cAUCGAUguCGGCUUGCccgaacccuacaGCGuGCCg -3' miRNA: 3'- gauaUAGCUAacGCCGAACG------------UGC-CGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 19864 | 0.67 | 0.903354 |
Target: 5'- -----gCGaAUUGCGGCUU-UACGGUCa -3' miRNA: 3'- gauauaGC-UAACGCCGAAcGUGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 5382 | 0.67 | 0.896233 |
Target: 5'- -cAUGUCGAUgacGCGGgUU-C-CGGCCg -3' miRNA: 3'- gaUAUAGCUAa--CGCCgAAcGuGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 4534 | 0.67 | 0.896233 |
Target: 5'- cCUGaAUCgGGUUGCaGCaUGCcCGGCCc -3' miRNA: 3'- -GAUaUAG-CUAACGcCGaACGuGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 25608 | 0.67 | 0.896233 |
Target: 5'- --cUGUCGGccGCuGCUgcgccgGCACGGCUg -3' miRNA: 3'- gauAUAGCUaaCGcCGAa-----CGUGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 38009 | 0.67 | 0.888828 |
Target: 5'- -----gCGGUUGCGGC--GCACGGg- -3' miRNA: 3'- gauauaGCUAACGCCGaaCGUGCCgg -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 30137 | 0.67 | 0.888828 |
Target: 5'- gUGUcgCGAccgGCGGCgugaUGgGCGGCg -3' miRNA: 3'- gAUAuaGCUaa-CGCCGa---ACgUGCCGg -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 55588 | 0.67 | 0.881145 |
Target: 5'- -cGUGUCGAggccgGCaGGCUccUGCcCGGCg -3' miRNA: 3'- gaUAUAGCUaa---CG-CCGA--ACGuGCCGg -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 31633 | 0.67 | 0.881145 |
Target: 5'- -cAUAUCGAgaccGCgGGCgcGCgugGCGGCCa -3' miRNA: 3'- gaUAUAGCUaa--CG-CCGaaCG---UGCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 50674 | 0.67 | 0.873188 |
Target: 5'- ---cGUCGAggGCGGCgUGC-CaGCCc -3' miRNA: 3'- gauaUAGCUaaCGCCGaACGuGcCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 13168 | 0.67 | 0.873188 |
Target: 5'- gCUGcGUCGGcgGCGGUcgGCGgCGGCg -3' miRNA: 3'- -GAUaUAGCUaaCGCCGaaCGU-GCCGg -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 5337 | 0.68 | 0.829576 |
Target: 5'- -gAUAUCGccUGCcGCUggUGCGgCGGCCa -3' miRNA: 3'- gaUAUAGCuaACGcCGA--ACGU-GCCGG- -5' |
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12459 | 3' | -50.6 | NC_003324.1 | + | 30318 | 0.68 | 0.856485 |
Target: 5'- -gGUGUCGGcgGUGGCgucgcucgGCGCGuGCUg -3' miRNA: 3'- gaUAUAGCUaaCGCCGaa------CGUGC-CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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