Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12465 | 3' | -50.4 | NC_003324.1 | + | 40129 | 1.1 | 0.003354 |
Target: 5'- uCUCGACAUCGAUGCCGAAGGAAUCGGc -3' miRNA: 3'- -GAGCUGUAGCUACGGCUUCCUUAGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 21597 | 0.81 | 0.253461 |
Target: 5'- uUCGGCGUCGA-GCCGAacAGGAAcCGGa -3' miRNA: 3'- gAGCUGUAGCUaCGGCU--UCCUUaGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 40204 | 0.78 | 0.387336 |
Target: 5'- uUCGGCAUCGAUGUCGAGacgcuGGAAUggUGGa -3' miRNA: 3'- gAGCUGUAGCUACGGCUU-----CCUUA--GCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 8739 | 0.75 | 0.560091 |
Target: 5'- uCUUGGCggCGGUGUCGGAGaAGUCGGu -3' miRNA: 3'- -GAGCUGuaGCUACGGCUUCcUUAGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 40264 | 0.75 | 0.561189 |
Target: 5'- gUCGGCAUCGcucggcugauguuccUGUCGAAGGuGUCGGa -3' miRNA: 3'- gAGCUGUAGCu--------------ACGGCUUCCuUAGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 6853 | 0.72 | 0.682424 |
Target: 5'- -cCGACAUCGAUGUCccAGGAcgCGa -3' miRNA: 3'- gaGCUGUAGCUACGGcuUCCUuaGCc -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 34495 | 0.72 | 0.726171 |
Target: 5'- -cCGACAUcacCGAUGCCGA-GGAcggCGGc -3' miRNA: 3'- gaGCUGUA---GCUACGGCUuCCUua-GCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 26200 | 0.71 | 0.758009 |
Target: 5'- gUCGAgAUCaGGUaGCCGAAGGcccguUCGGg -3' miRNA: 3'- gAGCUgUAG-CUA-CGGCUUCCuu---AGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 56142 | 0.7 | 0.798545 |
Target: 5'- aUCGGCcgCuuuaugGCCGAGGG-AUCGGa -3' miRNA: 3'- gAGCUGuaGcua---CGGCUUCCuUAGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 34419 | 0.7 | 0.787652 |
Target: 5'- cCUCGGCAUCGGUgaugucgGCCGAcGGcacAUUGGc -3' miRNA: 3'- -GAGCUGUAGCUA-------CGGCUuCCu--UAGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 13897 | 0.7 | 0.808259 |
Target: 5'- aUCG-CAUCGGUGUuguucucggagaCGAAGGAGUUGa -3' miRNA: 3'- gAGCuGUAGCUACG------------GCUUCCUUAGCc -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 18258 | 0.69 | 0.836218 |
Target: 5'- -aCGACAgagccgCGAccGCUGAcGGAAUCGGc -3' miRNA: 3'- gaGCUGUa-----GCUa-CGGCUuCCUUAGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 6072 | 0.69 | 0.87036 |
Target: 5'- aUCGcugGUCGAgaucGCCGAGGGcaAGUCGGu -3' miRNA: 3'- gAGCug-UAGCUa---CGGCUUCC--UUAGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 3246 | 0.68 | 0.878278 |
Target: 5'- aUCGGCG-CGGUGCCGGugcuuUCGGa -3' miRNA: 3'- gAGCUGUaGCUACGGCUuccuuAGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 38408 | 0.68 | 0.87593 |
Target: 5'- cCUCGugAUCGAgacgcccaaagacaUGCUGcAAGGGcUUGGg -3' miRNA: 3'- -GAGCugUAGCU--------------ACGGC-UUCCUuAGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 33794 | 0.68 | 0.885934 |
Target: 5'- gCUCGAguCAUCGucaCCGAAGG-AUUGGa -3' miRNA: 3'- -GAGCU--GUAGCuacGGCUUCCuUAGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 22973 | 0.68 | 0.885934 |
Target: 5'- aUCGGCAUgGAUGCUGAguaugAGGca-CGGu -3' miRNA: 3'- gAGCUGUAgCUACGGCU-----UCCuuaGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 23770 | 0.67 | 0.911898 |
Target: 5'- gUCGACAUCGAUgugaagcccggcgcGCCGu-GGAuacCGGc -3' miRNA: 3'- gAGCUGUAGCUA--------------CGGCuuCCUua-GCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 5397 | 0.67 | 0.920111 |
Target: 5'- gUCGGCGUCGAggaucaUGUCGAu-GAcgCGGg -3' miRNA: 3'- gAGCUGUAGCU------ACGGCUucCUuaGCC- -5' |
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12465 | 3' | -50.4 | NC_003324.1 | + | 32704 | 0.67 | 0.926103 |
Target: 5'- cCUCGACAgUGAUGUCGAGuGGAGc--- -3' miRNA: 3'- -GAGCUGUaGCUACGGCUU-CCUUagcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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