Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12465 | 3' | -50.4 | NC_003324.1 | + | 54343 | 0.67 | 0.931809 |
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Target: 5'- gCUCGGCGcUGAUGCCGGuccauGGcAAcCGGu -3' miRNA: 3'- -GAGCUGUaGCUACGGCUu----CC-UUaGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 30726 | 0.67 | 0.93723 |
|
Target: 5'- uCUUGGCGUCGGccgccUGCUGcucGGGAGUgaCGGa -3' miRNA: 3'- -GAGCUGUAGCU-----ACGGCu--UCCUUA--GCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 10245 | 0.67 | 0.931809 |
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Target: 5'- aUCG-CAgCGAUGCCGgAAGGuc-CGGa -3' miRNA: 3'- gAGCuGUaGCUACGGC-UUCCuuaGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 45285 | 0.67 | 0.928419 |
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Target: 5'- gCUCGGCGaucugugcuuccaguUCGA-GCCGGcgcuGGGAUCGa -3' miRNA: 3'- -GAGCUGU---------------AGCUaCGGCUu---CCUUAGCc -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 38369 | 0.67 | 0.920111 |
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Target: 5'- gUCGcCGUCG-UGCgGGAGGAAgUGGu -3' miRNA: 3'- gAGCuGUAGCuACGgCUUCCUUaGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 41836 | 0.67 | 0.93723 |
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Target: 5'- gUCGACAUCGAgcaaaugGuuGggGGugccgUGGc -3' miRNA: 3'- gAGCUGUAGCUa------CggCuuCCuua--GCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 1388 | 0.66 | 0.956106 |
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Target: 5'- -gUGACAUCGAUgcGCCGAcGGugcUGGg -3' miRNA: 3'- gaGCUGUAGCUA--CGGCUuCCuuaGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 13636 | 0.66 | 0.951802 |
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Target: 5'- aUCGGCAUcCGAU-CCGAAG---UCGGg -3' miRNA: 3'- gAGCUGUA-GCUAcGGCUUCcuuAGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 23309 | 0.66 | 0.951802 |
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Target: 5'- --gGACAUCcGUGuuGAAGGuGUCGu -3' miRNA: 3'- gagCUGUAGcUACggCUUCCuUAGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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