Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12465 | 3' | -50.4 | NC_003324.1 | + | 38369 | 0.67 | 0.920111 |
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Target: 5'- gUCGcCGUCG-UGCgGGAGGAAgUGGu -3' miRNA: 3'- gAGCuGUAGCuACGgCUUCCUUaGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 38408 | 0.68 | 0.87593 |
|
Target: 5'- cCUCGugAUCGAgacgcccaaagacaUGCUGcAAGGGcUUGGg -3' miRNA: 3'- -GAGCugUAGCU--------------ACGGC-UUCCUuAGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 40129 | 1.1 | 0.003354 |
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Target: 5'- uCUCGACAUCGAUGCCGAAGGAAUCGGc -3' miRNA: 3'- -GAGCUGUAGCUACGGCUUCCUUAGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 40204 | 0.78 | 0.387336 |
|
Target: 5'- uUCGGCAUCGAUGUCGAGacgcuGGAAUggUGGa -3' miRNA: 3'- gAGCUGUAGCUACGGCUU-----CCUUA--GCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 40264 | 0.75 | 0.561189 |
|
Target: 5'- gUCGGCAUCGcucggcugauguuccUGUCGAAGGuGUCGGa -3' miRNA: 3'- gAGCUGUAGCu--------------ACGGCUUCCuUAGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 41836 | 0.67 | 0.93723 |
|
Target: 5'- gUCGACAUCGAgcaaaugGuuGggGGugccgUGGc -3' miRNA: 3'- gAGCUGUAGCUa------CggCuuCCuua--GCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 45285 | 0.67 | 0.928419 |
|
Target: 5'- gCUCGGCGaucugugcuuccaguUCGA-GCCGGcgcuGGGAUCGa -3' miRNA: 3'- -GAGCUGU---------------AGCUaCGGCUu---CCUUAGCc -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 54343 | 0.67 | 0.931809 |
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Target: 5'- gCUCGGCGcUGAUGCCGGuccauGGcAAcCGGu -3' miRNA: 3'- -GAGCUGUaGCUACGGCUu----CC-UUaGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 56142 | 0.7 | 0.798545 |
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Target: 5'- aUCGGCcgCuuuaugGCCGAGGG-AUCGGa -3' miRNA: 3'- gAGCUGuaGcua---CGGCUUCCuUAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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