Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12465 | 3' | -50.4 | NC_003324.1 | + | 13897 | 0.7 | 0.808259 |
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Target: 5'- aUCG-CAUCGGUGUuguucucggagaCGAAGGAGUUGa -3' miRNA: 3'- gAGCuGUAGCUACG------------GCUUCCUUAGCc -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 13636 | 0.66 | 0.951802 |
|
Target: 5'- aUCGGCAUcCGAU-CCGAAG---UCGGg -3' miRNA: 3'- gAGCUGUA-GCUAcGGCUUCcuuAGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 10245 | 0.67 | 0.931809 |
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Target: 5'- aUCG-CAgCGAUGCCGgAAGGuc-CGGa -3' miRNA: 3'- gAGCuGUaGCUACGGC-UUCCuuaGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 8739 | 0.75 | 0.560091 |
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Target: 5'- uCUUGGCggCGGUGUCGGAGaAGUCGGu -3' miRNA: 3'- -GAGCUGuaGCUACGGCUUCcUUAGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 6853 | 0.72 | 0.682424 |
|
Target: 5'- -cCGACAUCGAUGUCccAGGAcgCGa -3' miRNA: 3'- gaGCUGUAGCUACGGcuUCCUuaGCc -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 6072 | 0.69 | 0.87036 |
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Target: 5'- aUCGcugGUCGAgaucGCCGAGGGcaAGUCGGu -3' miRNA: 3'- gAGCug-UAGCUa---CGGCUUCC--UUAGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 5397 | 0.67 | 0.920111 |
|
Target: 5'- gUCGGCGUCGAggaucaUGUCGAu-GAcgCGGg -3' miRNA: 3'- gAGCUGUAGCU------ACGGCUucCUuaGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 3246 | 0.68 | 0.878278 |
|
Target: 5'- aUCGGCG-CGGUGCCGGugcuuUCGGa -3' miRNA: 3'- gAGCUGUaGCUACGGCUuccuuAGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 1388 | 0.66 | 0.956106 |
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Target: 5'- -gUGACAUCGAUgcGCCGAcGGugcUGGg -3' miRNA: 3'- gaGCUGUAGCUA--CGGCUuCCuuaGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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