Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 12465 | 3' | -50.4 | NC_003324.1 | + | 13897 | 0.7 | 0.808259 |
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Target: 5'- aUCG-CAUCGGUGUuguucucggagaCGAAGGAGUUGa -3' miRNA: 3'- gAGCuGUAGCUACG------------GCUUCCUUAGCc -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 26200 | 0.71 | 0.758009 |
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Target: 5'- gUCGAgAUCaGGUaGCCGAAGGcccguUCGGg -3' miRNA: 3'- gAGCUgUAG-CUA-CGGCUUCCuu---AGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 34495 | 0.72 | 0.726171 |
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Target: 5'- -cCGACAUcacCGAUGCCGA-GGAcggCGGc -3' miRNA: 3'- gaGCUGUA---GCUACGGCUuCCUua-GCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 6853 | 0.72 | 0.682424 |
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Target: 5'- -cCGACAUCGAUGUCccAGGAcgCGa -3' miRNA: 3'- gaGCUGUAGCUACGGcuUCCUuaGCc -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 40264 | 0.75 | 0.561189 |
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Target: 5'- gUCGGCAUCGcucggcugauguuccUGUCGAAGGuGUCGGa -3' miRNA: 3'- gAGCUGUAGCu--------------ACGGCUUCCuUAGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 8739 | 0.75 | 0.560091 |
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Target: 5'- uCUUGGCggCGGUGUCGGAGaAGUCGGu -3' miRNA: 3'- -GAGCUGuaGCUACGGCUUCcUUAGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 40204 | 0.78 | 0.387336 |
|
Target: 5'- uUCGGCAUCGAUGUCGAGacgcuGGAAUggUGGa -3' miRNA: 3'- gAGCUGUAGCUACGGCUU-----CCUUA--GCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 21597 | 0.81 | 0.253461 |
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Target: 5'- uUCGGCGUCGA-GCCGAacAGGAAcCGGa -3' miRNA: 3'- gAGCUGUAGCUaCGGCU--UCCUUaGCC- -5' |
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| 12465 | 3' | -50.4 | NC_003324.1 | + | 40129 | 1.1 | 0.003354 |
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Target: 5'- uCUCGACAUCGAUGCCGAAGGAAUCGGc -3' miRNA: 3'- -GAGCUGUAGCUACGGCUUCCUUAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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