Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12466 | 5' | -50.6 | NC_003324.1 | + | 2674 | 0.7 | 0.815012 |
Target: 5'- uUCCAgCGCGguggccgcuucggcaAgggCACGAUGUCGGcgGCGGc -3' miRNA: 3'- -AGGUaGCGC---------------Ua--GUGCUACAGCU--UGCC- -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 4125 | 0.66 | 0.952658 |
Target: 5'- uUCC-UCGUGAUguCGAUGaaGAGCGc -3' miRNA: 3'- -AGGuAGCGCUAguGCUACagCUUGCc -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 7386 | 0.69 | 0.829841 |
Target: 5'- ----aCGaCGGUCACGGUGUUGGGCuGGa -3' miRNA: 3'- agguaGC-GCUAGUGCUACAGCUUG-CC- -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 7849 | 0.69 | 0.861991 |
Target: 5'- cCCGUUGCGAUCGCGccucccugcgaggcGUGcucUCGGACa- -3' miRNA: 3'- aGGUAGCGCUAGUGC--------------UAC---AGCUUGcc -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 8671 | 0.67 | 0.933009 |
Target: 5'- aCCGcCGCcaaGAUCugGAUcUCGggUGGa -3' miRNA: 3'- aGGUaGCG---CUAGugCUAcAGCuuGCC- -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 12888 | 0.69 | 0.838836 |
Target: 5'- gCCAUCGuCGAUgggCGCGGUGUUGGuCGa -3' miRNA: 3'- aGGUAGC-GCUA---GUGCUACAGCUuGCc -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 12958 | 0.69 | 0.864457 |
Target: 5'- cCCAUCGaCGAUgGCGAaGUCGuGAUGa -3' miRNA: 3'- aGGUAGC-GCUAgUGCUaCAGC-UUGCc -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 18341 | 0.78 | 0.40149 |
Target: 5'- uUCCGUCaGCGGUCGCGGcucUGUCGucGAUGGc -3' miRNA: 3'- -AGGUAG-CGCUAGUGCU---ACAGC--UUGCC- -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 19207 | 0.76 | 0.469626 |
Target: 5'- cUCCAUCGCcuuGcgCACGuUGUCGAGCaGGa -3' miRNA: 3'- -AGGUAGCG---CuaGUGCuACAGCUUG-CC- -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 20493 | 0.7 | 0.820634 |
Target: 5'- aCCGcUUGCGAUCgACGAugacggcuccguUGUCGAauACGGc -3' miRNA: 3'- aGGU-AGCGCUAG-UGCU------------ACAGCU--UGCC- -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 21503 | 0.72 | 0.675599 |
Target: 5'- cCCGUCGCGuugcUCAag--GUCGGACGGc -3' miRNA: 3'- aGGUAGCGCu---AGUgcuaCAGCUUGCC- -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 22007 | 0.66 | 0.938338 |
Target: 5'- -gCAUCGaCGAg-GCGcAUGUCGAGCGc -3' miRNA: 3'- agGUAGC-GCUagUGC-UACAGCUUGCc -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 22951 | 0.67 | 0.921506 |
Target: 5'- gUCCAgucUC-CGGUgGCGAUG-CGAGCGa -3' miRNA: 3'- -AGGU---AGcGCUAgUGCUACaGCUUGCc -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 24750 | 0.68 | 0.872514 |
Target: 5'- cCCucgaGUCGCuugcgcagGAUUACGAUGUCGGugGu -3' miRNA: 3'- aGG----UAGCG--------CUAGUGCUACAGCUugCc -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 28480 | 0.66 | 0.956885 |
Target: 5'- aUCCAUC-CGAgCcCGcUGUUGGGCGGu -3' miRNA: 3'- -AGGUAGcGCUaGuGCuACAGCUUGCC- -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 28849 | 0.67 | 0.933009 |
Target: 5'- aUCCAgaagaagcuggUCGCGGUCAgaGAUG-CGGagcGCGGc -3' miRNA: 3'- -AGGU-----------AGCGCUAGUg-CUACaGCU---UGCC- -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 29540 | 0.68 | 0.895139 |
Target: 5'- uUCCGUCGUGAUCucaGAUG-C--ACGGg -3' miRNA: 3'- -AGGUAGCGCUAGug-CUACaGcuUGCC- -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 30106 | 0.66 | 0.956885 |
Target: 5'- uUCgGUCaGCGAUUGCGAaggGUCGAcGCGc -3' miRNA: 3'- -AGgUAG-CGCUAGUGCUa--CAGCU-UGCc -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 32490 | 0.76 | 0.459523 |
Target: 5'- gCC-UCgGCGAUCACGGUGUCG-ACGa -3' miRNA: 3'- aGGuAG-CGCUAGUGCUACAGCuUGCc -5' |
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12466 | 5' | -50.6 | NC_003324.1 | + | 33329 | 0.67 | 0.933009 |
Target: 5'- gUCGUCGUGAcCuuGAUGgaCGGACGGa -3' miRNA: 3'- aGGUAGCGCUaGugCUACa-GCUUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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