Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12467 | 5' | -52.7 | NC_003324.1 | + | 39261 | 0.66 | 0.846017 |
Target: 5'- cGGUugCcGCCGugGcuGCGCcagaagGUGCCGAu -3' miRNA: 3'- -CCAugGaCGGUugU--UGCG------CACGGUUu -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 29989 | 0.66 | 0.837032 |
Target: 5'- --cGCCguccagcaugGCCAGCAGCGC--GCCGAAg -3' miRNA: 3'- ccaUGGa---------CGGUUGUUGCGcaCGGUUU- -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 29101 | 0.66 | 0.837032 |
Target: 5'- cGGUACCgcGUCGA--GCGCGcUGCUAAGg -3' miRNA: 3'- -CCAUGGa-CGGUUguUGCGC-ACGGUUU- -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 14500 | 0.66 | 0.827819 |
Target: 5'- cGGUGCa-GCCAACAcuGCGCG-GCa--- -3' miRNA: 3'- -CCAUGgaCGGUUGU--UGCGCaCGguuu -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 42175 | 0.66 | 0.818388 |
Target: 5'- cGUACUUGCCgAACGACGgcacUGcGCCGGAg -3' miRNA: 3'- cCAUGGACGG-UUGUUGC----GCaCGGUUU- -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 166 | 0.66 | 0.808749 |
Target: 5'- gGGgauauuCCUGUaGACGGCGCGcGCCAu- -3' miRNA: 3'- -CCau----GGACGgUUGUUGCGCaCGGUuu -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 3278 | 0.66 | 0.807774 |
Target: 5'- aGG-GCCUGCCAcucaucaaaGCAaucccauucaucgGCGCGgUGCCGGu -3' miRNA: 3'- -CCaUGGACGGU---------UGU-------------UGCGC-ACGGUUu -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 33684 | 0.66 | 0.798914 |
Target: 5'- --cGCC-GUCGGCAACGCGcUGCgCAAAg -3' miRNA: 3'- ccaUGGaCGGUUGUUGCGC-ACG-GUUU- -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 25860 | 0.66 | 0.798914 |
Target: 5'- cGGUACC-GCCAagACGugGC-UGCUGAc -3' miRNA: 3'- -CCAUGGaCGGU--UGUugCGcACGGUUu -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 17383 | 0.67 | 0.778704 |
Target: 5'- cGUACCggcuugggUGCCAugAucucaagucCGCGUGCCAc- -3' miRNA: 3'- cCAUGG--------ACGGUugUu--------GCGCACGGUuu -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 34506 | 0.67 | 0.768351 |
Target: 5'- uGUGCCgucgGCCGACAucAC-CGaUGCCGAGg -3' miRNA: 3'- cCAUGGa---CGGUUGU--UGcGC-ACGGUUU- -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 24326 | 0.67 | 0.75785 |
Target: 5'- --cACCaUGCCGGCAACGCG-GUCc-- -3' miRNA: 3'- ccaUGG-ACGGUUGUUGCGCaCGGuuu -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 33595 | 0.67 | 0.75785 |
Target: 5'- ---cCCUGCUGcuuugcGCAGCGCGuUGCCGAc -3' miRNA: 3'- ccauGGACGGU------UGUUGCGC-ACGGUUu -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 17789 | 0.67 | 0.747213 |
Target: 5'- cGGUGa-UGCCGGCGAUcugguaGCGUGCCu-- -3' miRNA: 3'- -CCAUggACGGUUGUUG------CGCACGGuuu -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 49341 | 0.68 | 0.736452 |
Target: 5'- cGUGCUgugcggcGCCAACAGCG-GUGUCGAc -3' miRNA: 3'- cCAUGGa------CGGUUGUUGCgCACGGUUu -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 16246 | 0.69 | 0.68011 |
Target: 5'- cGGUGCCcgccucggUGCCAGCGaugccugGCGCG-GCCc-- -3' miRNA: 3'- -CCAUGG--------ACGGUUGU-------UGCGCaCGGuuu -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 47410 | 0.69 | 0.677865 |
Target: 5'- --gGCCUGCCAGgcggagcacagcuuCGACGCcUGCCAc- -3' miRNA: 3'- ccaUGGACGGUU--------------GUUGCGcACGGUuu -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 5332 | 0.69 | 0.669991 |
Target: 5'- --cGCCUGCCGcUggUGCGgcgGCCAAc -3' miRNA: 3'- ccaUGGACGGUuGuuGCGCa--CGGUUu -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 12755 | 0.69 | 0.658712 |
Target: 5'- --aAUCgaGCCAGCGAUGCGUucGCCAGAc -3' miRNA: 3'- ccaUGGa-CGGUUGUUGCGCA--CGGUUU- -5' |
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12467 | 5' | -52.7 | NC_003324.1 | + | 21076 | 0.69 | 0.658712 |
Target: 5'- -cUGCCUugucgGCCGccGCGGCGCGUGCUg-- -3' miRNA: 3'- ccAUGGA-----CGGU--UGUUGCGCACGGuuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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