Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12467 | 5' | -52.7 | NC_003324.1 | + | 22607 | 0.7 | 0.610055 |
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Target: 5'- uGGcUGCCUGCCGcguugccgagccccGCGACGCGcUGCaUAGAg -3' miRNA: 3'- -CC-AUGGACGGU--------------UGUUGCGC-ACG-GUUU- -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 14572 | 0.7 | 0.57964 |
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Target: 5'- gGGUcgcgGCCUGCCGGCGcaaGCG-GCCGGc -3' miRNA: 3'- -CCA----UGGACGGUUGUug-CGCaCGGUUu -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 43794 | 0.7 | 0.56845 |
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Target: 5'- gGGUGCCgggaacGCCGGCAagGCGCGUGaaaucaCGGAu -3' miRNA: 3'- -CCAUGGa-----CGGUUGU--UGCGCACg-----GUUU- -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 42560 | 0.7 | 0.56845 |
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Target: 5'- cGUGCUUGCC-GCAAUGacCGUGCCGGc -3' miRNA: 3'- cCAUGGACGGuUGUUGC--GCACGGUUu -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 30219 | 0.71 | 0.524322 |
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Target: 5'- uGGUACCaGCCAGC-ACGC--GCCGAGc -3' miRNA: 3'- -CCAUGGaCGGUUGuUGCGcaCGGUUU- -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 41523 | 1.09 | 0.001726 |
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Target: 5'- cGGUACCUGCCAACAACGCGUGCCAAAc -3' miRNA: 3'- -CCAUGGACGGUUGUUGCGCACGGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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