Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12467 | 5' | -52.7 | NC_003324.1 | + | 14572 | 0.7 | 0.57964 |
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Target: 5'- gGGUcgcgGCCUGCCGGCGcaaGCG-GCCGGc -3' miRNA: 3'- -CCA----UGGACGGUUGUug-CGCaCGGUUu -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 14500 | 0.66 | 0.827819 |
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Target: 5'- cGGUGCa-GCCAACAcuGCGCG-GCa--- -3' miRNA: 3'- -CCAUGgaCGGUUGU--UGCGCaCGguuu -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 12755 | 0.69 | 0.658712 |
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Target: 5'- --aAUCgaGCCAGCGAUGCGUucGCCAGAc -3' miRNA: 3'- ccaUGGa-CGGUUGUUGCGCA--CGGUUU- -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 5332 | 0.69 | 0.669991 |
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Target: 5'- --cGCCUGCCGcUggUGCGgcgGCCAAc -3' miRNA: 3'- ccaUGGACGGUuGuuGCGCa--CGGUUu -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 3278 | 0.66 | 0.807774 |
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Target: 5'- aGG-GCCUGCCAcucaucaaaGCAaucccauucaucgGCGCGgUGCCGGu -3' miRNA: 3'- -CCaUGGACGGU---------UGU-------------UGCGC-ACGGUUu -5' |
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| 12467 | 5' | -52.7 | NC_003324.1 | + | 166 | 0.66 | 0.808749 |
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Target: 5'- gGGgauauuCCUGUaGACGGCGCGcGCCAu- -3' miRNA: 3'- -CCau----GGACGgUUGUUGCGCaCGGUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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