miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12468 5' -51.8 NC_003324.1 + 44163 0.66 0.869978
Target:  5'- gAUCUCGCUGcgcaagcaggacaccGaCGAgcgGAUGGAGCAa -3'
miRNA:   3'- aUGGAGCGAC---------------CaGCUaa-CUAUCUCGU- -5'
12468 5' -51.8 NC_003324.1 + 25841 0.68 0.761303
Target:  5'- -uCCUCaaGCUGGaugcggCGcgUGGUGGAGCGa -3'
miRNA:   3'- auGGAG--CGACCa-----GCuaACUAUCUCGU- -5'
12468 5' -51.8 NC_003324.1 + 41030 0.69 0.728909
Target:  5'- cGCCUCGCUGuUCGAgugUGAgccGGCGa -3'
miRNA:   3'- aUGGAGCGACcAGCUa--ACUaucUCGU- -5'
12468 5' -51.8 NC_003324.1 + 56482 0.71 0.616062
Target:  5'- aGCCUUGUcGGUCGAUUGGUcaaacgaggauGAGCGc -3'
miRNA:   3'- aUGGAGCGaCCAGCUAACUAu----------CUCGU- -5'
12468 5' -51.8 NC_003324.1 + 35926 0.71 0.604674
Target:  5'- cGCCaggCGCaGGUCGAaucccUGAUGGAGCu -3'
miRNA:   3'- aUGGa--GCGaCCAGCUa----ACUAUCUCGu -5'
12468 5' -51.8 NC_003324.1 + 6067 0.76 0.346103
Target:  5'- cUGCCaUCGCUGGUCGAgaucgccgAGGGCAa -3'
miRNA:   3'- -AUGG-AGCGACCAGCUaacua---UCUCGU- -5'
12468 5' -51.8 NC_003324.1 + 41797 1.08 0.002482
Target:  5'- uUACCUCGCUGGUCGAUUGAUAGAGCAa -3'
miRNA:   3'- -AUGGAGCGACCAGCUAACUAUCUCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.