Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12468 | 5' | -51.8 | NC_003324.1 | + | 44163 | 0.66 | 0.869978 |
Target: 5'- gAUCUCGCUGcgcaagcaggacaccGaCGAgcgGAUGGAGCAa -3' miRNA: 3'- aUGGAGCGAC---------------CaGCUaa-CUAUCUCGU- -5' |
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12468 | 5' | -51.8 | NC_003324.1 | + | 25841 | 0.68 | 0.761303 |
Target: 5'- -uCCUCaaGCUGGaugcggCGcgUGGUGGAGCGa -3' miRNA: 3'- auGGAG--CGACCa-----GCuaACUAUCUCGU- -5' |
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12468 | 5' | -51.8 | NC_003324.1 | + | 41030 | 0.69 | 0.728909 |
Target: 5'- cGCCUCGCUGuUCGAgugUGAgccGGCGa -3' miRNA: 3'- aUGGAGCGACcAGCUa--ACUaucUCGU- -5' |
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12468 | 5' | -51.8 | NC_003324.1 | + | 56482 | 0.71 | 0.616062 |
Target: 5'- aGCCUUGUcGGUCGAUUGGUcaaacgaggauGAGCGc -3' miRNA: 3'- aUGGAGCGaCCAGCUAACUAu----------CUCGU- -5' |
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12468 | 5' | -51.8 | NC_003324.1 | + | 35926 | 0.71 | 0.604674 |
Target: 5'- cGCCaggCGCaGGUCGAaucccUGAUGGAGCu -3' miRNA: 3'- aUGGa--GCGaCCAGCUa----ACUAUCUCGu -5' |
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12468 | 5' | -51.8 | NC_003324.1 | + | 6067 | 0.76 | 0.346103 |
Target: 5'- cUGCCaUCGCUGGUCGAgaucgccgAGGGCAa -3' miRNA: 3'- -AUGG-AGCGACCAGCUaacua---UCUCGU- -5' |
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12468 | 5' | -51.8 | NC_003324.1 | + | 41797 | 1.08 | 0.002482 |
Target: 5'- uUACCUCGCUGGUCGAUUGAUAGAGCAa -3' miRNA: 3'- -AUGGAGCGACCAGCUAACUAUCUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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