miRNA display CGI


Results 21 - 24 of 24 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12472 3' -57.4 NC_003324.1 + 4693 0.66 0.600209
Target:  5'- uCAGCGGCAucauguuGGCguUcuUCGgaucCCGGGCCGa -3'
miRNA:   3'- -GUUGUCGUu------CCGguA--AGC----GGCCCGGU- -5'
12472 3' -57.4 NC_003324.1 + 3321 0.67 0.535419
Target:  5'- gGAUuGCAGGGCUuagucgCGCUGGGCg- -3'
miRNA:   3'- gUUGuCGUUCCGGuaa---GCGGCCCGgu -5'
12472 3' -57.4 NC_003324.1 + 3162 0.66 0.589294
Target:  5'- gAAUGGCAAGaGCgGgagCGCCGugcGGCCAu -3'
miRNA:   3'- gUUGUCGUUC-CGgUaa-GCGGC---CCGGU- -5'
12472 3' -57.4 NC_003324.1 + 614 0.68 0.473251
Target:  5'- gGACAuCAAGGCCuggcaGCUGGGCUu -3'
miRNA:   3'- gUUGUcGUUCCGGuaag-CGGCCCGGu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.