Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12472 | 5' | -56.7 | NC_003324.1 | + | 43201 | 0.7 | 0.413638 |
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Target: 5'- cGGCAACCuGCgGGacuuuGGCAUUAUgCCCg -3' miRNA: 3'- -CCGUUGG-CGaCCgcu--CCGUAGUA-GGG- -5' |
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| 12472 | 5' | -56.7 | NC_003324.1 | + | 47071 | 0.66 | 0.629136 |
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Target: 5'- aGGCAGCC-Cga--GGGGCGUUAUCgCCg -3' miRNA: 3'- -CCGUUGGcGaccgCUCCGUAGUAG-GG- -5' |
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| 12472 | 5' | -56.7 | NC_003324.1 | + | 49048 | 0.66 | 0.629136 |
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Target: 5'- ----cCCGCggcGGCGGGGCAaucCAUCCg -3' miRNA: 3'- ccguuGGCGa--CCGCUCCGUa--GUAGGg -5' |
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| 12472 | 5' | -56.7 | NC_003324.1 | + | 53362 | 0.69 | 0.461341 |
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Target: 5'- --aAGCCGCgaccGGCGGGcGCgAUCAUUCCu -3' miRNA: 3'- ccgUUGGCGa---CCGCUC-CG-UAGUAGGG- -5' |
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| 12472 | 5' | -56.7 | NC_003324.1 | + | 57008 | 0.66 | 0.67678 |
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Target: 5'- cGCucuAUCGCUGGCGAGGCccgaaaggcggguggAUCuacgacgCUCg -3' miRNA: 3'- cCGu--UGGCGACCGCUCCG---------------UAGua-----GGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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