Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12474 | 3' | -51.6 | NC_003324.1 | + | 46736 | 0.66 | 0.932532 |
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Target: 5'- aGGgagagcACGGggCGcCGAGGUGUgc-CCCg -3' miRNA: 3'- -CCau----UGCCaaGC-GCUCCACAguaGGG- -5' |
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| 12474 | 3' | -51.6 | NC_003324.1 | + | 2859 | 0.66 | 0.921032 |
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Target: 5'- --cAACGGccgagaggUGCGAGGUGgucacgCAUCCg -3' miRNA: 3'- ccaUUGCCaa------GCGCUCCACa-----GUAGGg -5' |
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| 12474 | 3' | -51.6 | NC_003324.1 | + | 28591 | 0.66 | 0.921032 |
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Target: 5'- -uUGGCGGUUCGCGGcuuccgcccGcUGUCG-CCCu -3' miRNA: 3'- ccAUUGCCAAGCGCU---------CcACAGUaGGG- -5' |
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| 12474 | 3' | -51.6 | NC_003324.1 | + | 40604 | 0.66 | 0.921032 |
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Target: 5'- uGGUc-CGGUUCGCuGGG-GcCAUCCg -3' miRNA: 3'- -CCAuuGCCAAGCGcUCCaCaGUAGGg -5' |
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| 12474 | 3' | -51.6 | NC_003324.1 | + | 56742 | 0.66 | 0.921032 |
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Target: 5'- aGGgAACGuGacCGUGAGGUGagGUCUCu -3' miRNA: 3'- -CCaUUGC-CaaGCGCUCCACagUAGGG- -5' |
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| 12474 | 3' | -51.6 | NC_003324.1 | + | 22466 | 0.67 | 0.901696 |
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Target: 5'- uGGUGACGGaUUGgGAGcUGUCG-CCg -3' miRNA: 3'- -CCAUUGCCaAGCgCUCcACAGUaGGg -5' |
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| 12474 | 3' | -51.6 | NC_003324.1 | + | 17806 | 0.7 | 0.730169 |
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Target: 5'- uGGUAgcgugccucACGGcgCGCauucGGGGUGUCG-CCCu -3' miRNA: 3'- -CCAU---------UGCCaaGCG----CUCCACAGUaGGG- -5' |
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| 12474 | 3' | -51.6 | NC_003324.1 | + | 43047 | 1.16 | 0.001051 |
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Target: 5'- cGGUAACGGUUCGCGAGGUGUCAUCCCa -3' miRNA: 3'- -CCAUUGCCAAGCGCUCCACAGUAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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