Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12476 | 3' | -49.8 | NC_003324.1 | + | 9562 | 0.66 | 0.947687 |
Target: 5'- gUGGGucUGGGGuggcGAUGCcgcaaGUACCGGCa -3' miRNA: 3'- -ACUCuuACCUCu---UUACGa----CGUGGCUG- -5' |
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12476 | 3' | -49.8 | NC_003324.1 | + | 27190 | 0.66 | 0.947687 |
Target: 5'- cGAGAGcgaccgcgcUGGu---GUGCUGCGCCaGAUg -3' miRNA: 3'- aCUCUU---------ACCucuuUACGACGUGG-CUG- -5' |
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12476 | 3' | -49.8 | NC_003324.1 | + | 18913 | 0.66 | 0.937385 |
Target: 5'- cGAGAccggcgugAUGGAGAcug---GCGCCGACc -3' miRNA: 3'- aCUCU--------UACCUCUuuacgaCGUGGCUG- -5' |
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12476 | 3' | -49.8 | NC_003324.1 | + | 41818 | 0.66 | 0.931781 |
Target: 5'- aGAGcAAUGGcGAucgGCUGCACCc-- -3' miRNA: 3'- aCUC-UUACCuCUuuaCGACGUGGcug -5' |
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12476 | 3' | -49.8 | NC_003324.1 | + | 54082 | 0.66 | 0.928272 |
Target: 5'- cGAGAAUGuccgacauGGAAUGCaugaacacgcgucgGCGCCGACc -3' miRNA: 3'- aCUCUUACcu------CUUUACGa-------------CGUGGCUG- -5' |
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12476 | 3' | -49.8 | NC_003324.1 | + | 48699 | 0.67 | 0.919657 |
Target: 5'- ------cGGAGccGGAUGCggGCGCCGACc -3' miRNA: 3'- acucuuaCCUC--UUUACGa-CGUGGCUG- -5' |
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12476 | 3' | -49.8 | NC_003324.1 | + | 22149 | 0.67 | 0.899196 |
Target: 5'- -aAGAAgcaGGAGAAGg---GCGCCGACa -3' miRNA: 3'- acUCUUa--CCUCUUUacgaCGUGGCUG- -5' |
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12476 | 3' | -49.8 | NC_003324.1 | + | 25202 | 0.68 | 0.884072 |
Target: 5'- aUGAGAccaaGGAAGuUGcCUGCGCCGACu -3' miRNA: 3'- -ACUCUuaccUCUUU-AC-GACGUGGCUG- -5' |
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12476 | 3' | -49.8 | NC_003324.1 | + | 19412 | 0.68 | 0.859269 |
Target: 5'- aUGAGGucGGcuAGAUGCUGCGCCuuGGCc -3' miRNA: 3'- -ACUCUuaCCucUUUACGACGUGG--CUG- -5' |
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12476 | 3' | -49.8 | NC_003324.1 | + | 6786 | 0.69 | 0.832115 |
Target: 5'- gGAGAAaGGAGAA-UGCcGCcUCGACg -3' miRNA: 3'- aCUCUUaCCUCUUuACGaCGuGGCUG- -5' |
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12476 | 3' | -49.8 | NC_003324.1 | + | 35477 | 0.7 | 0.782375 |
Target: 5'- cGAGcGUGGGGAcggcGAUcGCcGUACCGGCu -3' miRNA: 3'- aCUCuUACCUCU----UUA-CGaCGUGGCUG- -5' |
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12476 | 3' | -49.8 | NC_003324.1 | + | 42417 | 0.71 | 0.717168 |
Target: 5'- -cGGAAUGGGGAAGUcGCucuaagucUGuCACCGGCg -3' miRNA: 3'- acUCUUACCUCUUUA-CG--------AC-GUGGCUG- -5' |
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12476 | 3' | -49.8 | NC_003324.1 | + | 45398 | 1.12 | 0.002139 |
Target: 5'- aUGAGAAUGGAGAAAUGCUGCACCGACg -3' miRNA: 3'- -ACUCUUACCUCUUUACGACGUGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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