Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12480 | 3' | -63.2 | NC_003324.1 | + | 45254 | 0.73 | 0.108745 |
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Target: 5'- cGAGGaGCGCCaAAUCGCGUCcuacuucgaacGCGCCAGg -3' miRNA: 3'- -UUCC-CGCGG-UUGGCGCAGc----------CGCGGUC- -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 48060 | 0.73 | 0.103019 |
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Target: 5'- -cGGGUcaGUCAG-CGCGUCGGUGCCGGu -3' miRNA: 3'- uuCCCG--CGGUUgGCGCAGCCGCGGUC- -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 48687 | 0.67 | 0.282452 |
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Target: 5'- -cGGGCGCCGACCuGCGugucgauuggguUCGGCuucuUCGGg -3' miRNA: 3'- uuCCCGCGGUUGG-CGC------------AGCCGc---GGUC- -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 48722 | 1.05 | 0.000345 |
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Target: 5'- aAAGGGCGCCAACCGCGUCGGCGCCAGc -3' miRNA: 3'- -UUCCCGCGGUUGGCGCAGCCGCGGUC- -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 48803 | 0.7 | 0.175271 |
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Target: 5'- --uGGCGCCGACgCG-GUUGGCGCCc- -3' miRNA: 3'- uucCCGCGGUUG-GCgCAGCCGCGGuc -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 49443 | 0.66 | 0.333379 |
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Target: 5'- cGAGGuCGCCcgaucgucgacACCGCuGUUGGCGCCGc -3' miRNA: 3'- -UUCCcGCGGu----------UGGCG-CAGCCGCGGUc -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 51310 | 0.66 | 0.325709 |
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Target: 5'- uAAGGGCGCgGACCa--UCG-CGCCGGa -3' miRNA: 3'- -UUCCCGCGgUUGGcgcAGCcGCGGUC- -5' |
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| 12480 | 3' | -63.2 | NC_003324.1 | + | 54960 | 0.66 | 0.31371 |
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Target: 5'- -cGGGCGCCGACauuCGCGagcauccgggccugCGGgGUCGGa -3' miRNA: 3'- uuCCCGCGGUUG---GCGCa-------------GCCgCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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