Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12482 | 5' | -56.3 | NC_003324.1 | + | 49508 | 1.05 | 0.001363 |
Target: 5'- gCGGCAUUCUGGCACUCUCGGCCGUAUc -3' miRNA: 3'- -GCCGUAAGACCGUGAGAGCCGGCAUA- -5' |
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12482 | 5' | -56.3 | NC_003324.1 | + | 14538 | 0.72 | 0.317259 |
Target: 5'- aGGCg--CUGGCGCUCaaGGCCGa-- -3' miRNA: 3'- gCCGuaaGACCGUGAGagCCGGCaua -5' |
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12482 | 5' | -56.3 | NC_003324.1 | + | 47754 | 0.7 | 0.384324 |
Target: 5'- aGGUAUUCUcGGCAC-Cg-GGCCGUGa -3' miRNA: 3'- gCCGUAAGA-CCGUGaGagCCGGCAUa -5' |
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12482 | 5' | -56.3 | NC_003324.1 | + | 38319 | 0.68 | 0.532484 |
Target: 5'- uCGGCAUUCcGGCGCUgaaagaggauUUCGGCaacuaCGUGUu -3' miRNA: 3'- -GCCGUAAGaCCGUGA----------GAGCCG-----GCAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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