Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12486 | 3' | -51.7 | NC_003324.1 | + | 24935 | 0.7 | 0.707624 |
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Target: 5'- cCGCCCgCGAGCAGauugccaagacGGCCGACcUUgUCGg -3' miRNA: 3'- -GUGGG-GUUUGUU-----------UCGGCUGaAGgAGC- -5' |
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| 12486 | 3' | -51.7 | NC_003324.1 | + | 13086 | 0.7 | 0.674322 |
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Target: 5'- cCGCCgCCGAcCGccGCCGACgcagCCUCGc -3' miRNA: 3'- -GUGG-GGUUuGUuuCGGCUGaa--GGAGC- -5' |
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| 12486 | 3' | -51.7 | NC_003324.1 | + | 38040 | 0.72 | 0.59567 |
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Target: 5'- cCGCCgaCCGAcACGAgacGGCgGACUUCCUCGc -3' miRNA: 3'- -GUGG--GGUU-UGUU---UCGgCUGAAGGAGC- -5' |
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| 12486 | 3' | -51.7 | NC_003324.1 | + | 30062 | 0.72 | 0.562243 |
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Target: 5'- aCGCCgCCGGucGCGAcaccGGCCGcugcgGCUUCCUCGa -3' miRNA: 3'- -GUGG-GGUU--UGUU----UCGGC-----UGAAGGAGC- -5' |
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| 12486 | 3' | -51.7 | NC_003324.1 | + | 37752 | 0.72 | 0.562243 |
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Target: 5'- gCAgCUUGAGCGGGaucGCCGACUUCCUCu -3' miRNA: 3'- -GUgGGGUUUGUUU---CGGCUGAAGGAGc -5' |
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| 12486 | 3' | -51.7 | NC_003324.1 | + | 50898 | 1.02 | 0.007855 |
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Target: 5'- -cCCCCAAACAAAGCCGACUUCCUCGu -3' miRNA: 3'- guGGGGUUUGUUUCGGCUGAAGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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