Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12486 | 3' | -51.7 | NC_003324.1 | + | 39656 | 0.69 | 0.761416 |
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Target: 5'- aGCCaCCGAGCGc-GCCcuCUUCCUCGa -3' miRNA: 3'- gUGG-GGUUUGUuuCGGcuGAAGGAGC- -5' |
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| 12486 | 3' | -51.7 | NC_003324.1 | + | 40186 | 0.66 | 0.896465 |
|
Target: 5'- aCAUCagCCGAGCGAuGCCGACgaagcgcUUCUCGa -3' miRNA: 3'- -GUGG--GGUUUGUUuCGGCUGa------AGGAGC- -5' |
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| 12486 | 3' | -51.7 | NC_003324.1 | + | 48932 | 0.69 | 0.761416 |
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Target: 5'- gACCUgGu-CAAGGCCGucGCUUCUUCGg -3' miRNA: 3'- gUGGGgUuuGUUUCGGC--UGAAGGAGC- -5' |
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| 12486 | 3' | -51.7 | NC_003324.1 | + | 49146 | 0.66 | 0.889141 |
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Target: 5'- gGCCCCGGuGCAGAGCCGGauggauugCCcCGc -3' miRNA: 3'- gUGGGGUU-UGUUUCGGCUgaa-----GGaGC- -5' |
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| 12486 | 3' | -51.7 | NC_003324.1 | + | 50898 | 1.02 | 0.007855 |
|
Target: 5'- -cCCCCAAACAAAGCCGACUUCCUCGu -3' miRNA: 3'- guGGGGUUUGUUUCGGCUGAAGGAGC- -5' |
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| 12486 | 3' | -51.7 | NC_003324.1 | + | 55499 | 0.67 | 0.848539 |
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Target: 5'- cCACgCCGGGCAgGAGCCuGCcggCCUCGa -3' miRNA: 3'- -GUGgGGUUUGU-UUCGGcUGaa-GGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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