Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12487 | 3' | -58 | NC_003324.1 | + | 5632 | 0.68 | 0.49685 |
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Target: 5'- gCGGcGAAGGUU-GCgGCGGCaACCGg -3' miRNA: 3'- gGCCaCUUCUAGuCGgCGCCGcUGGC- -5' |
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| 12487 | 3' | -58 | NC_003324.1 | + | 30168 | 0.67 | 0.547797 |
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Target: 5'- aCCGaaggcGUcGAGGA--AGCCGCaGCGGCCGg -3' miRNA: 3'- -GGC-----CA-CUUCUagUCGGCGcCGCUGGC- -5' |
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| 12487 | 3' | -58 | NC_003324.1 | + | 56146 | 0.67 | 0.564463 |
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Target: 5'- gCGGguucgccagccugGAAGccgaCGGCCGUGGCGACUu -3' miRNA: 3'- gGCCa------------CUUCua--GUCGGCGCCGCUGGc -5' |
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| 12487 | 3' | -58 | NC_003324.1 | + | 8690 | 0.67 | 0.579153 |
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Target: 5'- aCCGGUGAuGGUCGaaaCCGCGcccGUGACgCGg -3' miRNA: 3'- -GGCCACUuCUAGUc--GGCGC---CGCUG-GC- -5' |
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| 12487 | 3' | -58 | NC_003324.1 | + | 39964 | 0.66 | 0.589697 |
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Target: 5'- gCGcUGGAGAUCGGCaagGaaGCGGCCGa -3' miRNA: 3'- gGCcACUUCUAGUCGg--CgcCGCUGGC- -5' |
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| 12487 | 3' | -58 | NC_003324.1 | + | 25546 | 0.66 | 0.599215 |
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Target: 5'- cCCGGgacgagcaaagcaUGcucgccaaguGGGUCGGCUGgGGCGGCCu -3' miRNA: 3'- -GGCC-------------ACu---------UCUAGUCGGCgCCGCUGGc -5' |
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| 12487 | 3' | -58 | NC_003324.1 | + | 7377 | 0.66 | 0.600274 |
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Target: 5'- aCGGUGuugggcuGGAUCuGCgugauguaGCGaGCGACCGu -3' miRNA: 3'- gGCCACu------UCUAGuCGg-------CGC-CGCUGGC- -5' |
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| 12487 | 3' | -58 | NC_003324.1 | + | 7772 | 0.66 | 0.632123 |
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Target: 5'- gCCGGUGuuuccGGGGUCGGCgGUGugcaGCGACa- -3' miRNA: 3'- -GGCCAC-----UUCUAGUCGgCGC----CGCUGgc -5' |
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| 12487 | 3' | -58 | NC_003324.1 | + | 13163 | 0.75 | 0.194456 |
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Target: 5'- gUCGGcGgcGGUCGGCgGCGGCGguACCGg -3' miRNA: 3'- -GGCCaCuuCUAGUCGgCGCCGC--UGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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