Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12489 | 5' | -57.5 | NC_003324.1 | + | 40529 | 0.66 | 0.607446 |
Target: 5'- cGAuCGAGCGCGGCgaGaaGCCucUGGu -3' miRNA: 3'- uCU-GCUCGCGCUGgaCggCGGuuACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 5031 | 0.66 | 0.607446 |
Target: 5'- cGACuacGGCGUGAUCUcgaucgacaccGCCGCCAagGAc -3' miRNA: 3'- uCUGc--UCGCGCUGGA-----------CGGCGGUuaCU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 26534 | 0.66 | 0.596628 |
Target: 5'- uGAUGucGC-CGACaUUGCCGUCGAUGAa -3' miRNA: 3'- uCUGCu-CGcGCUG-GACGGCGGUUACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 15115 | 0.66 | 0.595547 |
Target: 5'- uGGCGuccuGCGCcaugaucGACCUGCCGUCGc--- -3' miRNA: 3'- uCUGCu---CGCG-------CUGGACGGCGGUuacu -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 17493 | 0.66 | 0.585839 |
Target: 5'- cGACGAGgGCGACgUGgCaCGCgGAcuUGAg -3' miRNA: 3'- uCUGCUCgCGCUGgAC-G-GCGgUU--ACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 1160 | 0.66 | 0.585839 |
Target: 5'- gGGGCGAGCGgGAa--GCCGCgAAaGAg -3' miRNA: 3'- -UCUGCUCGCgCUggaCGGCGgUUaCU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 52248 | 0.66 | 0.575087 |
Target: 5'- cGGCcAGUuCGuCCUGCCGCCGAaGAc -3' miRNA: 3'- uCUGcUCGcGCuGGACGGCGGUUaCU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 40314 | 0.66 | 0.575087 |
Target: 5'- cAGuCGAGCGgGAgCCaGCaccgaaGCCGGUGAa -3' miRNA: 3'- -UCuGCUCGCgCU-GGaCGg-----CGGUUACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 30361 | 0.66 | 0.568658 |
Target: 5'- cGGCGGcuuGgGCGACCgUGCCuuggcccgcaucaucGCCGAUGGu -3' miRNA: 3'- uCUGCU---CgCGCUGG-ACGG---------------CGGUUACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 16427 | 0.66 | 0.564382 |
Target: 5'- uGGACGA-CGCGAUCgacggcaacGCCGUCGcgGAc -3' miRNA: 3'- -UCUGCUcGCGCUGGa--------CGGCGGUuaCU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 43013 | 0.67 | 0.543137 |
Target: 5'- gGGACGAGacCGcCGGCgCagUGaCCGCCAAUGGu -3' miRNA: 3'- -UCUGCUC--GC-GCUG-G--AC-GGCGGUUACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 56883 | 0.67 | 0.543137 |
Target: 5'- cGACG-GCGUGACCgaauuccCCGgCAAUGGa -3' miRNA: 3'- uCUGCuCGCGCUGGac-----GGCgGUUACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 49261 | 0.67 | 0.532613 |
Target: 5'- cGAgGAGC-CGAUCgagGCUGUCGAUGGu -3' miRNA: 3'- uCUgCUCGcGCUGGa--CGGCGGUUACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 24915 | 0.67 | 0.532613 |
Target: 5'- aAGACGgccgaccuugucGGCGCGAUCcGCCuGCCGAa-- -3' miRNA: 3'- -UCUGC------------UCGCGCUGGaCGG-CGGUUacu -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 45534 | 0.67 | 0.522162 |
Target: 5'- -aACGGGaau-ACCUGCCGCCAGUGu -3' miRNA: 3'- ucUGCUCgcgcUGGACGGCGGUUACu -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 21258 | 0.67 | 0.521122 |
Target: 5'- aGGAUGAGCGgauugccCGACgCUGCCGCUu---- -3' miRNA: 3'- -UCUGCUCGC-------GCUG-GACGGCGGuuacu -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 14569 | 0.67 | 0.511792 |
Target: 5'- -cAUGGGuCGCGGCCUGCCGgCGc--- -3' miRNA: 3'- ucUGCUC-GCGCUGGACGGCgGUuacu -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 33519 | 0.67 | 0.511792 |
Target: 5'- cGGCGAGCGUuACacgGacaaCGCCGAUGAa -3' miRNA: 3'- uCUGCUCGCGcUGga-Cg---GCGGUUACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 57160 | 0.68 | 0.501509 |
Target: 5'- cGACuGAGUGCGaagccaaggGCCUGCCGCuCAcaGAc -3' miRNA: 3'- uCUG-CUCGCGC---------UGGACGGCG-GUuaCU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 30762 | 0.68 | 0.471228 |
Target: 5'- aAGcACGGGCGCGGuCCUG-CGCCGu--- -3' miRNA: 3'- -UC-UGCUCGCGCU-GGACgGCGGUuacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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