Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12489 | 5' | -57.5 | NC_003324.1 | + | 1160 | 0.66 | 0.585839 |
Target: 5'- gGGGCGAGCGgGAa--GCCGCgAAaGAg -3' miRNA: 3'- -UCUGCUCGCgCUggaCGGCGgUUaCU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 1535 | 0.7 | 0.360591 |
Target: 5'- cAGugGuGCGCGGCgCUaugGCC-CCGAUGAa -3' miRNA: 3'- -UCugCuCGCGCUG-GA---CGGcGGUUACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 1736 | 0.7 | 0.372537 |
Target: 5'- uGGACGAGaGCGAuccggucuggguaauCCcgGCCGCCAgaAUGAa -3' miRNA: 3'- -UCUGCUCgCGCU---------------GGa-CGGCGGU--UACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 5031 | 0.66 | 0.607446 |
Target: 5'- cGACuacGGCGUGAUCUcgaucgacaccGCCGCCAagGAc -3' miRNA: 3'- uCUGc--UCGCGCUGGA-----------CGGCGGUuaCU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 5543 | 0.69 | 0.422936 |
Target: 5'- gGGGCGGGCGaCGccGCCUGCCuacccagcGUCGAUGc -3' miRNA: 3'- -UCUGCUCGC-GC--UGGACGG--------CGGUUACu -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 8216 | 0.7 | 0.369096 |
Target: 5'- cAGuCGAGCGCGcAgCUGCCcgGCgGGUGAg -3' miRNA: 3'- -UCuGCUCGCGC-UgGACGG--CGgUUACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 12424 | 0.68 | 0.471228 |
Target: 5'- uGcCGAGCGCGGCaaUGCCGaCAAUGu -3' miRNA: 3'- uCuGCUCGCGCUGg-ACGGCgGUUACu -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 12704 | 0.69 | 0.395425 |
Target: 5'- cAGAUauGCGCcguuGAUCUGcCCGCCGAUGAu -3' miRNA: 3'- -UCUGcuCGCG----CUGGAC-GGCGGUUACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 14569 | 0.67 | 0.511792 |
Target: 5'- -cAUGGGuCGCGGCCUGCCGgCGc--- -3' miRNA: 3'- ucUGCUC-GCGCUGGACGGCgGUuacu -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 15115 | 0.66 | 0.595547 |
Target: 5'- uGGCGuccuGCGCcaugaucGACCUGCCGUCGc--- -3' miRNA: 3'- uCUGCu---CGCG-------CUGGACGGCGGUuacu -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 16427 | 0.66 | 0.564382 |
Target: 5'- uGGACGA-CGCGAUCgacggcaacGCCGUCGcgGAc -3' miRNA: 3'- -UCUGCUcGCGCUGGa--------CGGCGGUuaCU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 17493 | 0.66 | 0.585839 |
Target: 5'- cGACGAGgGCGACgUGgCaCGCgGAcuUGAg -3' miRNA: 3'- uCUGCUCgCGCUGgAC-G-GCGgUU--ACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 18334 | 0.69 | 0.422936 |
Target: 5'- -aGCGGuCGCGGCuCUGUCGUCGAUGGc -3' miRNA: 3'- ucUGCUcGCGCUG-GACGGCGGUUACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 18423 | 0.79 | 0.10112 |
Target: 5'- aAGGCGAcgcuggucauggucuGCGCGACCuUGCCGCCGGUcGGg -3' miRNA: 3'- -UCUGCU---------------CGCGCUGG-ACGGCGGUUA-CU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 18897 | 0.68 | 0.461341 |
Target: 5'- gAGACuGGCGcCGACCUucGCCGCgCcAUGGa -3' miRNA: 3'- -UCUGcUCGC-GCUGGA--CGGCG-GuUACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 20581 | 0.72 | 0.291023 |
Target: 5'- uGGCGAGCGgGAUCcggugcuguggcgccGUCGCCAGUGAc -3' miRNA: 3'- uCUGCUCGCgCUGGa--------------CGGCGGUUACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 21258 | 0.67 | 0.521122 |
Target: 5'- aGGAUGAGCGgauugccCGACgCUGCCGCUu---- -3' miRNA: 3'- -UCUGCUCGC-------GCUG-GACGGCGGuuacu -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 23186 | 0.7 | 0.369096 |
Target: 5'- cGGCGAGCGCGGuaccgcUCUGCaucgugCGCCAGaUGAa -3' miRNA: 3'- uCUGCUCGCGCU------GGACG------GCGGUU-ACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 23628 | 0.74 | 0.213903 |
Target: 5'- cGcCGAGCGCG-CCcGCCGCCAcgcuGUGGg -3' miRNA: 3'- uCuGCUCGCGCuGGaCGGCGGU----UACU- -5' |
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12489 | 5' | -57.5 | NC_003324.1 | + | 24915 | 0.67 | 0.532613 |
Target: 5'- aAGACGgccgaccuugucGGCGCGAUCcGCCuGCCGAa-- -3' miRNA: 3'- -UCUGC------------UCGCGCUGGaCGG-CGGUUacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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