Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12495 | 3' | -58.4 | NC_003324.1 | + | 9728 | 0.66 | 0.587043 |
Target: 5'- cCCAGcGCguUGGCGuuGCCgGCGGUGuuGGCc -3' miRNA: 3'- -GGUC-CG--ACCGCu-UGGgCGCUACc-UCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 35925 | 0.79 | 0.092763 |
Target: 5'- gCCAGGCgcaGGuCGAAUCCcUGAUGGAGCu -3' miRNA: 3'- -GGUCCGa--CC-GCUUGGGcGCUACCUCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 28904 | 0.77 | 0.118758 |
Target: 5'- aCAGGCUGGCgucaagcgcaauGAGCgCGUGAugUGGGGCa -3' miRNA: 3'- gGUCCGACCG------------CUUGgGCGCU--ACCUCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 15261 | 0.75 | 0.172898 |
Target: 5'- aCGGGCgGGUuugaucugcgGAACCgGCGAUGGAGa -3' miRNA: 3'- gGUCCGaCCG----------CUUGGgCGCUACCUCg -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 24994 | 0.75 | 0.177511 |
Target: 5'- aCCGGGC-GGCGuGCUgGCGAUGGuGGUg -3' miRNA: 3'- -GGUCCGaCCGCuUGGgCGCUACC-UCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 3445 | 0.72 | 0.260714 |
Target: 5'- aCCAGGCUGccguuCGAuccAUCCGUGAagGGAGCc -3' miRNA: 3'- -GGUCCGACc----GCU---UGGGCGCUa-CCUCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 41820 | 0.7 | 0.373074 |
Target: 5'- --uGGUUGGgGGugCCGUGGcgugaucgcaUGGAGCg -3' miRNA: 3'- gguCCGACCgCUugGGCGCU----------ACCUCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 42483 | 0.69 | 0.399156 |
Target: 5'- gCUGGcGCUGuuGCGGACCCGCGAccaGGAuaccGCg -3' miRNA: 3'- -GGUC-CGAC--CGCUUGGGCGCUa--CCU----CG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 26991 | 0.69 | 0.408106 |
Target: 5'- -uGGGCgaaGGuCGAACCCuucuuccaucGCGAcaUGGAGCc -3' miRNA: 3'- ggUCCGa--CC-GCUUGGG----------CGCU--ACCUCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 50933 | 0.68 | 0.463354 |
Target: 5'- gCCAuGCUgucgcugGGCGAACCgccugcaGCGAUGuGGGCg -3' miRNA: 3'- -GGUcCGA-------CCGCUUGGg------CGCUAC-CUCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 2863 | 0.67 | 0.514101 |
Target: 5'- aCCAGGC-GGUaGACCUGCcaGAUcGGGCg -3' miRNA: 3'- -GGUCCGaCCGcUUGGGCG--CUAcCUCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 20404 | 0.66 | 0.544992 |
Target: 5'- --cGGCgaUGGCGAACCC-CGAccGGaAGCu -3' miRNA: 3'- gguCCG--ACCGCUUGGGcGCUa-CC-UCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 57040 | 0.66 | 0.576458 |
Target: 5'- -uGGGCUGGgGAGCggCgGCGAgGGcGCg -3' miRNA: 3'- ggUCCGACCgCUUG--GgCGCUaCCuCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 56071 | 1.12 | 0.000329 |
Target: 5'- uCCAGGCUGGCGAACCCGCGAUGGAGCu -3' miRNA: 3'- -GGUCCGACCGCUUGGGCGCUACCUCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 45364 | 0.66 | 0.587043 |
Target: 5'- gCAGGC-GGCGAGCa-GCGA-GG-GCc -3' miRNA: 3'- gGUCCGaCCGCUUGggCGCUaCCuCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 26873 | 0.66 | 0.587043 |
Target: 5'- uUCAGGCUcaagGGUGAcGCCgggcuccauguCGCGAUGGAa- -3' miRNA: 3'- -GGUCCGA----CCGCU-UGG-----------GCGCUACCUcg -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 51157 | 0.66 | 0.576458 |
Target: 5'- uUCAGGCaGGUGAccugccgcgucaACCUGaCGAgcgGGGGUu -3' miRNA: 3'- -GGUCCGaCCGCU------------UGGGC-GCUa--CCUCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 21119 | 0.66 | 0.570128 |
Target: 5'- gUCGGGCUGGCGcuugcgcagcaucucGGCCUcgucagugaGCGAgcgGGcGCg -3' miRNA: 3'- -GGUCCGACCGC---------------UUGGG---------CGCUa--CCuCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 17716 | 0.67 | 0.514101 |
Target: 5'- aUCAGGCUGGCGAuACCCaucaGCaGGUuGaAGCc -3' miRNA: 3'- -GGUCCGACCGCU-UGGG----CG-CUAcC-UCG- -5' |
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12495 | 3' | -58.4 | NC_003324.1 | + | 417 | 0.67 | 0.483948 |
Target: 5'- -uGGGCUGGCgcaucccgGAGCCCGCGAa----- -3' miRNA: 3'- ggUCCGACCG--------CUUGGGCGCUaccucg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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