Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12495 | 3' | -58.4 | NC_003324.1 | + | 25114 | 0.68 | 0.474085 |
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Target: 5'- gCCGGGguagagguucUUGGCGAugCCGcCGGUGacgcgaucGAGCu -3' miRNA: 3'- -GGUCC----------GACCGCUugGGC-GCUAC--------CUCG- -5' |
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| 12495 | 3' | -58.4 | NC_003324.1 | + | 25843 | 0.68 | 0.464324 |
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Target: 5'- cUCAaGCUGGaugcGGCgCGUGGUGGAGCg -3' miRNA: 3'- -GGUcCGACCgc--UUGgGCGCUACCUCG- -5' |
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| 12495 | 3' | -58.4 | NC_003324.1 | + | 55037 | 0.68 | 0.445124 |
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Target: 5'- gCAGGCaaGGCGGACggcugggcugCCGCGAcGGcGCa -3' miRNA: 3'- gGUCCGa-CCGCUUG----------GGCGCUaCCuCG- -5' |
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| 12495 | 3' | -58.4 | NC_003324.1 | + | 42864 | 0.69 | 0.408106 |
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Target: 5'- uCgAGGCUgccGGCcGGCCCGCGA-GGAucuGCg -3' miRNA: 3'- -GgUCCGA---CCGcUUGGGCGCUaCCU---CG- -5' |
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| 12495 | 3' | -58.4 | NC_003324.1 | + | 45948 | 0.69 | 0.399156 |
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Target: 5'- gCAGGCUGGaaAGCCUcCGGgaGGAGCu -3' miRNA: 3'- gGUCCGACCgcUUGGGcGCUa-CCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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