Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12495 | 3' | -58.4 | NC_003324.1 | + | 17716 | 0.67 | 0.514101 |
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Target: 5'- aUCAGGCUGGCGAuACCCaucaGCaGGUuGaAGCc -3' miRNA: 3'- -GGUCCGACCGCU-UGGG----CG-CUAcC-UCG- -5' |
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| 12495 | 3' | -58.4 | NC_003324.1 | + | 21119 | 0.66 | 0.570128 |
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Target: 5'- gUCGGGCUGGCGcuugcgcagcaucucGGCCUcgucagugaGCGAgcgGGcGCg -3' miRNA: 3'- -GGUCCGACCGC---------------UUGGG---------CGCUa--CCuCG- -5' |
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| 12495 | 3' | -58.4 | NC_003324.1 | + | 51157 | 0.66 | 0.576458 |
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Target: 5'- uUCAGGCaGGUGAccugccgcgucaACCUGaCGAgcgGGGGUu -3' miRNA: 3'- -GGUCCGaCCGCU------------UGGGC-GCUa--CCUCG- -5' |
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| 12495 | 3' | -58.4 | NC_003324.1 | + | 26873 | 0.66 | 0.587043 |
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Target: 5'- uUCAGGCUcaagGGUGAcGCCgggcuccauguCGCGAUGGAa- -3' miRNA: 3'- -GGUCCGA----CCGCU-UGG-----------GCGCUACCUcg -5' |
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| 12495 | 3' | -58.4 | NC_003324.1 | + | 56071 | 1.12 | 0.000329 |
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Target: 5'- uCCAGGCUGGCGAACCCGCGAUGGAGCu -3' miRNA: 3'- -GGUCCGACCGCUUGGGCGCUACCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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