miRNA: 3'- -GGUCCGaCCGCuUGGgCGCUACC-UCG- -5'
miRNA: 3'- gGUCCGaCCG----------CUUGGgCGCUACCUCg -5'
miRNA: 3'- gGUCCGACCG------------CUUGgGCGCU--ACCUCG- -5'
miRNA: 3'- -GGUCCGa--CC-GCUUGGGcGCUACCUCG- -5'
miRNA: 3'- -GGUCCGACCGCUUGGGCGCUACCUCG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12495 | 3' | -58.4 | NC_003324.1 | + | 24994 | 0.75 | 0.177511 |
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Target: 5'- aCCGGGC-GGCGuGCUgGCGAUGGuGGUg -3' miRNA: 3'- -GGUCCGaCCGCuUGGgCGCUACC-UCG- -5' |
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| 12495 | 3' | -58.4 | NC_003324.1 | + | 15261 | 0.75 | 0.172898 |
|
Target: 5'- aCGGGCgGGUuugaucugcgGAACCgGCGAUGGAGa -3' miRNA: 3'- gGUCCGaCCG----------CUUGGgCGCUACCUCg -5' |
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| 12495 | 3' | -58.4 | NC_003324.1 | + | 28904 | 0.77 | 0.118758 |
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Target: 5'- aCAGGCUGGCgucaagcgcaauGAGCgCGUGAugUGGGGCa -3' miRNA: 3'- gGUCCGACCG------------CUUGgGCGCU--ACCUCG- -5' |
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| 12495 | 3' | -58.4 | NC_003324.1 | + | 35925 | 0.79 | 0.092763 |
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Target: 5'- gCCAGGCgcaGGuCGAAUCCcUGAUGGAGCu -3' miRNA: 3'- -GGUCCGa--CC-GCUUGGGcGCUACCUCG- -5' |
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| 12495 | 3' | -58.4 | NC_003324.1 | + | 56071 | 1.12 | 0.000329 |
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Target: 5'- uCCAGGCUGGCGAACCCGCGAUGGAGCu -3' miRNA: 3'- -GGUCCGACCGCUUGGGCGCUACCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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