Results 1 - 20 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12497 | 3' | -49.4 | NC_003324.1 | + | 49411 | 0.65 | 0.957251 |
Target: 5'- cGcCGCACAGCAcgUGcagauacucacgauGUA-GCGAUGCg -3' miRNA: 3'- aC-GCGUGUUGUaaAC--------------CGUaCGCUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 14514 | 0.66 | 0.954207 |
Target: 5'- -cUGCGCGGCAg--GGCGUccGCGAcaagGCg -3' miRNA: 3'- acGCGUGUUGUaaaCCGUA--CGCUa---CG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 20659 | 0.66 | 0.954207 |
Target: 5'- aGUGCuuCGACca--GGCG-GCGAUGCu -3' miRNA: 3'- aCGCGu-GUUGuaaaCCGUaCGCUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 4271 | 0.66 | 0.954207 |
Target: 5'- aUGCGcCACGGCGUUuccgccggUGGCAa--GAUGUc -3' miRNA: 3'- -ACGC-GUGUUGUAA--------ACCGUacgCUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 5280 | 0.66 | 0.949608 |
Target: 5'- cGCGCcaGCGAUAgaggGGUAaGCGAggGCa -3' miRNA: 3'- aCGCG--UGUUGUaaa-CCGUaCGCUa-CG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 57338 | 0.66 | 0.949608 |
Target: 5'- aGCGCaAUAGCAggcUGGCcgcuUGCGAcccuugGCg -3' miRNA: 3'- aCGCG-UGUUGUaa-ACCGu---ACGCUa-----CG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 19007 | 0.66 | 0.949608 |
Target: 5'- gGCuGCAC-GCGgcUGGCcucgaacGCGAUGCg -3' miRNA: 3'- aCG-CGUGuUGUaaACCGua-----CGCUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 48742 | 0.66 | 0.944713 |
Target: 5'- aUGCGCAgcCAGCGUcuccUGGCucAUGcCGAUGa -3' miRNA: 3'- -ACGCGU--GUUGUAa---ACCG--UAC-GCUACg -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 24287 | 0.66 | 0.944713 |
Target: 5'- cUGgGCACGGCGgagcUUGGCGaagGCGucGCg -3' miRNA: 3'- -ACgCGUGUUGUa---AACCGUa--CGCuaCG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 43036 | 0.66 | 0.944713 |
Target: 5'- cUGUGCACAugGCGUcaaGGCAUGgGAc-- -3' miRNA: 3'- -ACGCGUGU--UGUAaa-CCGUACgCUacg -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 35403 | 0.66 | 0.939516 |
Target: 5'- cGCGCGCAugGUUUccucGCccuCGGUGCg -3' miRNA: 3'- aCGCGUGUugUAAAc---CGuacGCUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 30005 | 0.67 | 0.934014 |
Target: 5'- gGUGCACuc----UGGCGacGCGAUGCu -3' miRNA: 3'- aCGCGUGuuguaaACCGUa-CGCUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 7332 | 0.67 | 0.934014 |
Target: 5'- cGCGCGCcgag---GGCAcgcUGaCGAUGCg -3' miRNA: 3'- aCGCGUGuuguaaaCCGU---AC-GCUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 42202 | 0.67 | 0.928207 |
Target: 5'- cGCG-GCGGCA-UUGGCgacgaGUGCGAaGCg -3' miRNA: 3'- aCGCgUGUUGUaAACCG-----UACGCUaCG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 41764 | 0.67 | 0.928207 |
Target: 5'- gGCGCAUcgcuACcgUUGGCAcagUGCGcgucgGCu -3' miRNA: 3'- aCGCGUGu---UGuaAACCGU---ACGCua---CG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 19146 | 0.67 | 0.922092 |
Target: 5'- aGCGCgACGACAaagGGCAaaUGgGcUGCa -3' miRNA: 3'- aCGCG-UGUUGUaaaCCGU--ACgCuACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 22995 | 0.67 | 0.922092 |
Target: 5'- cGCGCACucuucuuucGGCcgUaucGGCAUG-GAUGCu -3' miRNA: 3'- aCGCGUG---------UUGuaAa--CCGUACgCUACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 28551 | 0.67 | 0.922092 |
Target: 5'- cGCGCuCAACA--UGGCG-GCGuuaGCa -3' miRNA: 3'- aCGCGuGUUGUaaACCGUaCGCua-CG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 44826 | 0.67 | 0.922092 |
Target: 5'- aGgGgGCGGCGUcaUUGGCGUGCccgcUGCu -3' miRNA: 3'- aCgCgUGUUGUA--AACCGUACGcu--ACG- -5' |
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12497 | 3' | -49.4 | NC_003324.1 | + | 7775 | 0.67 | 0.922092 |
Target: 5'- cGCGC-CggUGUUUccggGGUcgGCGGUGUg -3' miRNA: 3'- aCGCGuGuuGUAAA----CCGuaCGCUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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