Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12512 | 3' | -54.4 | NC_003345.1 | + | 32479 | 0.66 | 0.829141 |
Target: 5'- cGAUgcggUGAGCCAC-CCGAGcGACcucggacucgauaAGCUc -3' miRNA: 3'- -CUGa---ACUCGGUGuGGCUCuCUG-------------UCGG- -5' |
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12512 | 3' | -54.4 | NC_003345.1 | + | 58456 | 0.66 | 0.83003 |
Target: 5'- uGugUUGGGCCACuCCuuGAGAGAacuucgAGCg -3' miRNA: 3'- -CugAACUCGGUGuGG--CUCUCUg-----UCGg -5' |
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12512 | 3' | -54.4 | NC_003345.1 | + | 22022 | 0.66 | 0.838817 |
Target: 5'- ----cGAGUCuuuACACCGAGguAGAgAGCCu -3' miRNA: 3'- cugaaCUCGG---UGUGGCUC--UCUgUCGG- -5' |
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12512 | 3' | -54.4 | NC_003345.1 | + | 38537 | 0.66 | 0.838817 |
Target: 5'- aACUUGGuaaucgaCGCAUCGAGGGuuuCGGCCa -3' miRNA: 3'- cUGAACUcg-----GUGUGGCUCUCu--GUCGG- -5' |
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12512 | 3' | -54.4 | NC_003345.1 | + | 33636 | 0.66 | 0.847393 |
Target: 5'- cGACUcGAaCUAUAUCGAGuuuGACAGCUc -3' miRNA: 3'- -CUGAaCUcGGUGUGGCUCu--CUGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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