Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1252 | 3' | -61 | NC_001317.1 | + | 16781 | 0.69 | 0.160208 |
Target: 5'- cGCCAGCGCU-CCGAgCUGAUGcaGGCc -3' miRNA: 3'- uCGGUCGCGGcGGCUgGACUAC--CUGc -5' |
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1252 | 3' | -61 | NC_001317.1 | + | 28762 | 0.69 | 0.164677 |
Target: 5'- cAGCCGGgGCgaaGCCcugcACCUGAUGGcCGg -3' miRNA: 3'- -UCGGUCgCGg--CGGc---UGGACUACCuGC- -5' |
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1252 | 3' | -61 | NC_001317.1 | + | 17302 | 0.69 | 0.173954 |
Target: 5'- --gCAGCGCUGCUGACCg---GGAUGa -3' miRNA: 3'- ucgGUCGCGGCGGCUGGacuaCCUGC- -5' |
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1252 | 3' | -61 | NC_001317.1 | + | 16290 | 0.68 | 0.193911 |
Target: 5'- cGGCCuGuCGaCCGauGACCUGAUGG-CGg -3' miRNA: 3'- -UCGGuC-GC-GGCggCUGGACUACCuGC- -5' |
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1252 | 3' | -61 | NC_001317.1 | + | 14699 | 0.66 | 0.294575 |
Target: 5'- cAGCCAGCGCgaGCUgauggucaucuGGCCUGAUuuccucgcaugGGAUa -3' miRNA: 3'- -UCGGUCGCGg-CGG-----------CUGGACUA-----------CCUGc -5' |
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1252 | 3' | -61 | NC_001317.1 | + | 446 | 1.07 | 0.000167 |
Target: 5'- aAGCCAGCGCCGCCGACCUGAUGGACGc -3' miRNA: 3'- -UCGGUCGCGGCGGCUGGACUACCUGC- -5' |
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1252 | 3' | -61 | NC_001317.1 | + | 2476 | 0.7 | 0.155848 |
Target: 5'- uGCUcGCGCCGCCGGugguuUCUGGUGG-Ca -3' miRNA: 3'- uCGGuCGCGGCGGCU-----GGACUACCuGc -5' |
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1252 | 3' | -61 | NC_001317.1 | + | 2612 | 0.68 | 0.199204 |
Target: 5'- gAGCugCAGCGCUGCaugguCGACgUGAUGGAa- -3' miRNA: 3'- -UCG--GUCGCGGCG-----GCUGgACUACCUgc -5' |
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1252 | 3' | -61 | NC_001317.1 | + | 2095 | 0.67 | 0.239869 |
Target: 5'- aAGCCAGUcaCGCCGAUaUUGcAUGGGCGa -3' miRNA: 3'- -UCGGUCGcgGCGGCUG-GAC-UACCUGC- -5' |
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1252 | 3' | -61 | NC_001317.1 | + | 5577 | 0.67 | 0.252698 |
Target: 5'- cGGCCAGCaGCUuaCGGCCaacGAUGGcaACGa -3' miRNA: 3'- -UCGGUCG-CGGcgGCUGGa--CUACC--UGC- -5' |
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1252 | 3' | -61 | NC_001317.1 | + | 24061 | 0.66 | 0.266086 |
Target: 5'- uGCCAGCGCuUGCCGuccuuACCUGcgauccagccGUGGcCGu -3' miRNA: 3'- uCGGUCGCG-GCGGC-----UGGAC----------UACCuGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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