Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12521 | 5' | -55.3 | NC_003345.1 | + | 43717 | 0.66 | 0.811904 |
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Target: 5'- cGAAGCAgGACACGacguaUCCccuCGcCGUAg -3' miRNA: 3'- uCUUCGUgCUGUGCg----AGGu--GCaGCGU- -5' |
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| 12521 | 5' | -55.3 | NC_003345.1 | + | 14602 | 0.66 | 0.773782 |
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Target: 5'- uGGGAGCGgucUGGaGCGUUCCACGgCGCu -3' miRNA: 3'- -UCUUCGU---GCUgUGCGAGGUGCaGCGu -5' |
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| 12521 | 5' | -55.3 | NC_003345.1 | + | 28594 | 0.67 | 0.753841 |
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Target: 5'- uAGAAGC-CGAgGCGCUgaaccUCugGUUGCc -3' miRNA: 3'- -UCUUCGuGCUgUGCGA-----GGugCAGCGu -5' |
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| 12521 | 5' | -55.3 | NC_003345.1 | + | 33224 | 0.67 | 0.753841 |
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Target: 5'- cGAAGguUGACcCGCUCUACGUgGa- -3' miRNA: 3'- uCUUCguGCUGuGCGAGGUGCAgCgu -5' |
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| 12521 | 5' | -55.3 | NC_003345.1 | + | 23889 | 0.67 | 0.743686 |
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Target: 5'- uGggGC-CGG-ACGCUCCACGgaggGCAa -3' miRNA: 3'- uCuuCGuGCUgUGCGAGGUGCag--CGU- -5' |
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| 12521 | 5' | -55.3 | NC_003345.1 | + | 69767 | 0.67 | 0.743686 |
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Target: 5'- aGGAcguGCGCGAC-CGCUCguaCugGUCgGCAa -3' miRNA: 3'- -UCUu--CGUGCUGuGCGAG---GugCAG-CGU- -5' |
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| 12521 | 5' | -55.3 | NC_003345.1 | + | 47548 | 0.67 | 0.712619 |
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Target: 5'- aGGggGCugACGACcUGUUCCGacgaGUCGCu -3' miRNA: 3'- -UCuuCG--UGCUGuGCGAGGUg---CAGCGu -5' |
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| 12521 | 5' | -55.3 | NC_003345.1 | + | 24906 | 0.69 | 0.616436 |
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Target: 5'- cGAGGgaACGACGCGCUCuCugGUUGa- -3' miRNA: 3'- uCUUCg-UGCUGUGCGAG-GugCAGCgu -5' |
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| 12521 | 5' | -55.3 | NC_003345.1 | + | 76789 | 0.74 | 0.360963 |
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Target: 5'- gGGAGcGCACGACACGCgagggCCGgGuUCGCu -3' miRNA: 3'- -UCUU-CGUGCUGUGCGa----GGUgC-AGCGu -5' |
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| 12521 | 5' | -55.3 | NC_003345.1 | + | 14765 | 1.08 | 0.001673 |
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Target: 5'- gAGAAGCACGACACGCUCCACGUCGCAc -3' miRNA: 3'- -UCUUCGUGCUGUGCGAGGUGCAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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