Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12522 | 3' | -53.5 | NC_003345.1 | + | 6952 | 0.66 | 0.878428 |
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Target: 5'- uCUCUGCgaaggcugucUCUCGGUGuGGCUCaagucuucggcugGUCGg -3' miRNA: 3'- -GAGACGau--------AGAGCCAC-CUGAG-------------CAGCg -5' |
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| 12522 | 3' | -53.5 | NC_003345.1 | + | 9889 | 0.66 | 0.871554 |
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Target: 5'- gUC-GCUGUCgcugUCGGUgGGGCUgGUUGUg -3' miRNA: 3'- gAGaCGAUAG----AGCCA-CCUGAgCAGCG- -5' |
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| 12522 | 3' | -53.5 | NC_003345.1 | + | 4553 | 0.67 | 0.838727 |
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Target: 5'- ---cGCcgGUCgUCGGuUGGugUCGUUGCu -3' miRNA: 3'- gagaCGa-UAG-AGCC-ACCugAGCAGCG- -5' |
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| 12522 | 3' | -53.5 | NC_003345.1 | + | 59479 | 0.7 | 0.701573 |
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Target: 5'- gCUCUGCUGg-UCGGUGGugGCUaccUCGCu -3' miRNA: 3'- -GAGACGAUagAGCCACC--UGAgc-AGCG- -5' |
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| 12522 | 3' | -53.5 | NC_003345.1 | + | 61332 | 0.7 | 0.680247 |
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Target: 5'- gCUCcGCaAUCUCGaUGGACUUGcCGCc -3' miRNA: 3'- -GAGaCGaUAGAGCcACCUGAGCaGCG- -5' |
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| 12522 | 3' | -53.5 | NC_003345.1 | + | 15100 | 1.11 | 0.001881 |
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Target: 5'- uCUCUGCUAUCUCGGUGGACUCGUCGCc -3' miRNA: 3'- -GAGACGAUAGAGCCACCUGAGCAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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