Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12524 | 5' | -50 | NC_003345.1 | + | 3373 | 0.66 | 0.975184 |
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Target: 5'- gGAGGgaaccggCGGAGaGAGCGgcGGaUUGCGAUg -3' miRNA: 3'- -CUCCa------GCCUUaCUCGC--UCaAAUGCUA- -5' |
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| 12524 | 5' | -50 | NC_003345.1 | + | 13041 | 0.69 | 0.896325 |
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Target: 5'- uAGGUCGGGAagGGGUGAGUcgGCa-- -3' miRNA: 3'- cUCCAGCCUUa-CUCGCUCAaaUGcua -5' |
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| 12524 | 5' | -50 | NC_003345.1 | + | 70775 | 0.69 | 0.889004 |
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Target: 5'- -cGGUCGGAGUGAcCGAGcauaUACGGc -3' miRNA: 3'- cuCCAGCCUUACUcGCUCaa--AUGCUa -5' |
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| 12524 | 5' | -50 | NC_003345.1 | + | 23228 | 0.7 | 0.865426 |
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Target: 5'- -cGGUCGGGGaGAGCGGGcgucaUGCGGUc -3' miRNA: 3'- cuCCAGCCUUaCUCGCUCaa---AUGCUA- -5' |
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| 12524 | 5' | -50 | NC_003345.1 | + | 47109 | 0.7 | 0.86043 |
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Target: 5'- -uGGUCGGuucaucGUGgcgaagcgaaacgagGGCGAGUUUGCGAa -3' miRNA: 3'- cuCCAGCCu-----UAC---------------UCGCUCAAAUGCUa -5' |
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| 12524 | 5' | -50 | NC_003345.1 | + | 16580 | 1.05 | 0.008397 |
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Target: 5'- gGAGGUCGGAAUGAGCGAGUUUACGAUu -3' miRNA: 3'- -CUCCAGCCUUACUCGCUCAAAUGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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