Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12525 | 3' | -53.1 | NC_003345.1 | + | 60300 | 0.66 | 0.89426 |
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Target: 5'- gCUA-CCUCGAUgcUCGCUa--UGGCCGGu -3' miRNA: 3'- -GAUaGGAGCUA--AGUGAgagGCCGGUC- -5' |
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| 12525 | 3' | -53.1 | NC_003345.1 | + | 63525 | 0.66 | 0.89426 |
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Target: 5'- -cAUCCUCGAU--GCUCUCuCGGagGGa -3' miRNA: 3'- gaUAGGAGCUAagUGAGAG-GCCggUC- -5' |
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| 12525 | 3' | -53.1 | NC_003345.1 | + | 6963 | 0.66 | 0.879662 |
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Target: 5'- gCUGUCuCUCGGUguggcUCAagUCUUCGGCUGGu -3' miRNA: 3'- -GAUAG-GAGCUA-----AGUg-AGAGGCCGGUC- -5' |
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| 12525 | 3' | -53.1 | NC_003345.1 | + | 52632 | 0.67 | 0.864064 |
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Target: 5'- -aGUCCUCcuuuugUCccgccCUCUCCGGUCGGg -3' miRNA: 3'- gaUAGGAGcua---AGu----GAGAGGCCGGUC- -5' |
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| 12525 | 3' | -53.1 | NC_003345.1 | + | 3885 | 0.71 | 0.656202 |
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Target: 5'- -cAUCCUcccaauaCGAUUC-CUCUCCGGCUu- -3' miRNA: 3'- gaUAGGA-------GCUAAGuGAGAGGCCGGuc -5' |
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| 12525 | 3' | -53.1 | NC_003345.1 | + | 4210 | 0.71 | 0.624597 |
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Target: 5'- -cAUCCUCGAUUCGgUCUuCCGGggcgUCGGg -3' miRNA: 3'- gaUAGGAGCUAAGUgAGA-GGCC----GGUC- -5' |
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| 12525 | 3' | -53.1 | NC_003345.1 | + | 66394 | 0.79 | 0.245827 |
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Target: 5'- --uUCCUCGAUUCcCUCaaUCCGGCCAc -3' miRNA: 3'- gauAGGAGCUAAGuGAG--AGGCCGGUc -5' |
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| 12525 | 3' | -53.1 | NC_003345.1 | + | 16856 | 1.08 | 0.002934 |
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Target: 5'- gCUAUCCUCGAUUCACUCUCCGGCCAGc -3' miRNA: 3'- -GAUAGGAGCUAAGUGAGAGGCCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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