Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12533 | 5' | -53 | NC_003345.1 | + | 20780 | 0.7 | 0.706122 |
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Target: 5'- cCCUCGGCGuCGGGUaucuucccuuaaucUGGACGUGu-UCa -3' miRNA: 3'- -GGAGUCGCuGUUCG--------------ACCUGCGCuuAG- -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 16363 | 0.67 | 0.837467 |
|
Target: 5'- aCUCAcgaacGCGACcgcuucgagaggGAGCUGGAacgaGCGGAUg -3' miRNA: 3'- gGAGU-----CGCUG------------UUCGACCUg---CGCUUAg -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 14963 | 0.68 | 0.790242 |
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Target: 5'- cCCggCAGCGcgGCGGGCUucgcuccgaacacGGGCGaCGAGUCc -3' miRNA: 3'- -GGa-GUCGC--UGUUCGA-------------CCUGC-GCUUAG- -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 13990 | 0.73 | 0.524789 |
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Target: 5'- uCCUCAGCGugGucgaaguaggugaAGCUa-ACGCGAGUCu -3' miRNA: 3'- -GGAGUCGCugU-------------UCGAccUGCGCUUAG- -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 12935 | 0.68 | 0.799877 |
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Target: 5'- cCCUCgaAGCccaucacuugacgGGCGAGCUGGuCGauaGAAUCa -3' miRNA: 3'- -GGAG--UCG-------------CUGUUCGACCuGCg--CUUAG- -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 12670 | 0.71 | 0.66623 |
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Target: 5'- aCCUCAGCGGCGugAGCcGGuCGaacCGAGUUg -3' miRNA: 3'- -GGAGUCGCUGU--UCGaCCuGC---GCUUAG- -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 1479 | 0.75 | 0.407096 |
|
Target: 5'- uCCUUAGCGAC-AGCuaUGGAaaCGUGAAUCc -3' miRNA: 3'- -GGAGUCGCUGuUCG--ACCU--GCGCUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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