Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12533 | 5' | -53 | NC_003345.1 | + | 64365 | 0.66 | 0.906125 |
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Target: 5'- uCC-CAuauGCGACAAGUgGGGCcgaugaacgcgaaGCGAAUCa -3' miRNA: 3'- -GGaGU---CGCUGUUCGaCCUG-------------CGCUUAG- -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 65327 | 0.66 | 0.900088 |
|
Target: 5'- --gCGGUGACAGGCUcGACcuCGAAUCc -3' miRNA: 3'- ggaGUCGCUGUUCGAcCUGc-GCUUAG- -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 67278 | 0.7 | 0.719935 |
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Target: 5'- aCCUCGGCGGCAacgaaAGCgacGGugG-GAGUg -3' miRNA: 3'- -GGAGUCGCUGU-----UCGa--CCugCgCUUAg -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 68185 | 0.73 | 0.519555 |
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Target: 5'- aCCUCAGCGuCGuucagagaagcacggAGCUGGACGUGc--- -3' miRNA: 3'- -GGAGUCGCuGU---------------UCGACCUGCGCuuag -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 70207 | 0.67 | 0.838342 |
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Target: 5'- cUCUCGGUgagGACGAGCUGGcaaacuucucuaucuCGCGGAa- -3' miRNA: 3'- -GGAGUCG---CUGUUCGACCu--------------GCGCUUag -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 73513 | 0.7 | 0.687881 |
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Target: 5'- aCC-CGGCGGCGguGGCUGa--GCGAAUCu -3' miRNA: 3'- -GGaGUCGCUGU--UCGACcugCGCUUAG- -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 77015 | 0.67 | 0.846119 |
|
Target: 5'- gUCUCGGuCGGgGAGCUGGuCGagccaaucCGggUCg -3' miRNA: 3'- -GGAGUC-GCUgUUCGACCuGC--------GCuuAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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