Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12533 | 5' | -53 | NC_003345.1 | + | 33142 | 0.73 | 0.505013 |
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Target: 5'- cCCUCGGUGugGAGCUuccgGCGAAUCa -3' miRNA: 3'- -GGAGUCGCugUUCGAccugCGCUUAG- -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 29662 | 0.74 | 0.484552 |
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Target: 5'- -gUCAGCGAaAAGCUGGAgGCGGc-- -3' miRNA: 3'- ggAGUCGCUgUUCGACCUgCGCUuag -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 31445 | 0.75 | 0.444881 |
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Target: 5'- aCUUCAuuaucgguGCGACAAGCUGGACGU---UCg -3' miRNA: 3'- -GGAGU--------CGCUGUUCGACCUGCGcuuAG- -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 1479 | 0.75 | 0.407096 |
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Target: 5'- uCCUUAGCGAC-AGCuaUGGAaaCGUGAAUCc -3' miRNA: 3'- -GGAGUCGCUGuUCG--ACCU--GCGCUUAG- -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 46502 | 0.76 | 0.380112 |
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Target: 5'- uCCUgAGCGACAAGCUGGAgGaCGu--- -3' miRNA: 3'- -GGAgUCGCUGUUCGACCUgC-GCuuag -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 34300 | 0.82 | 0.173593 |
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Target: 5'- aCCUCAGCGACcAGCucaccgUGGACGUGAAg- -3' miRNA: 3'- -GGAGUCGCUGuUCG------ACCUGCGCUUag -5' |
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| 12533 | 5' | -53 | NC_003345.1 | + | 24268 | 1.13 | 0.001514 |
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Target: 5'- gCCUCAGCGACAAGCUGGACGCGAAUCa -3' miRNA: 3'- -GGAGUCGCUGUUCGACCUGCGCUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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