Results 21 - 30 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12577 | 3' | -51.1 | NC_003345.1 | + | 40181 | 0.66 | 0.964114 |
Target: 5'- gGCGAcGAGCGAgacuaccuccgaCGAgccgCGUucgAGCCGCUGu -3' miRNA: 3'- -CGCUuCUUGCU------------GUUa---GCA---UCGGCGGC- -5' |
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12577 | 3' | -51.1 | NC_003345.1 | + | 14598 | 0.66 | 0.963758 |
Target: 5'- aGCGGucuGGAgcguuccACGGCGcUCGUGGgUCGCCa -3' miRNA: 3'- -CGCU---UCU-------UGCUGUuAGCAUC-GGCGGc -5' |
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12577 | 3' | -51.1 | NC_003345.1 | + | 50440 | 0.66 | 0.960438 |
Target: 5'- gGCGAAGcuCGu--AUCGggagguggAGCCGCUGg -3' miRNA: 3'- -CGCUUCuuGCuguUAGCa-------UCGGCGGC- -5' |
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12577 | 3' | -51.1 | NC_003345.1 | + | 14516 | 0.66 | 0.960057 |
Target: 5'- cGgGAAGugGACGACGGccCGUAGCUcaggauaGCCGa -3' miRNA: 3'- -CgCUUC--UUGCUGUUa-GCAUCGG-------CGGC- -5' |
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12577 | 3' | -51.1 | NC_003345.1 | + | 10912 | 0.66 | 0.95651 |
Target: 5'- uCGggGAGgGAgcaguccuuaCAGUUGUcGCCGCUGg -3' miRNA: 3'- cGCuuCUUgCU----------GUUAGCAuCGGCGGC- -5' |
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12577 | 3' | -51.1 | NC_003345.1 | + | 3013 | 0.66 | 0.952324 |
Target: 5'- cCGggGAGuCGAacccCGgAGCCGCCGc -3' miRNA: 3'- cGCuuCUU-GCUguuaGCaUCGGCGGC- -5' |
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12577 | 3' | -51.1 | NC_003345.1 | + | 20471 | 0.66 | 0.952324 |
Target: 5'- cCGAAGGguuCGAgAGUCGcuucgaGGUCGCCGg -3' miRNA: 3'- cGCUUCUu--GCUgUUAGCa-----UCGGCGGC- -5' |
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12577 | 3' | -51.1 | NC_003345.1 | + | 31012 | 0.66 | 0.952324 |
Target: 5'- uUGAGGGuguuCGACcucUCGccGCCGCCGg -3' miRNA: 3'- cGCUUCUu---GCUGuu-AGCauCGGCGGC- -5' |
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12577 | 3' | -51.1 | NC_003345.1 | + | 73736 | 0.67 | 0.943161 |
Target: 5'- -aGAGGAcGCGAUGGggcgCGgAGCCGCUGa -3' miRNA: 3'- cgCUUCU-UGCUGUUa---GCaUCGGCGGC- -5' |
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12577 | 3' | -51.1 | NC_003345.1 | + | 71296 | 1.11 | 0.003443 |
Target: 5'- uGCGAAGAACGACAAUCGUAGCCGCCGu -3' miRNA: 3'- -CGCUUCUUGCUGUUAGCAUCGGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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