Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12581 | 3' | -47.5 | NC_003345.1 | + | 60697 | 0.71 | 0.937635 |
Target: 5'- gGAAGGCGcG-GGAUUCGGcacGAGUGCGa -3' miRNA: 3'- -CUUUUGC-CgCCUAAGCCa--UUCAUGCc -5' |
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12581 | 3' | -47.5 | NC_003345.1 | + | 61142 | 0.66 | 0.997082 |
Target: 5'- --cGGCGGCGGuaauggUCGGcGcucucggagugagacAGUACGGu -3' miRNA: 3'- cuuUUGCCGCCua----AGCCaU---------------UCAUGCC- -5' |
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12581 | 3' | -47.5 | NC_003345.1 | + | 64671 | 0.73 | 0.893719 |
Target: 5'- uGAGAGC-GCGGGUUCGGUAcGUAUc- -3' miRNA: 3'- -CUUUUGcCGCCUAAGCCAUuCAUGcc -5' |
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12581 | 3' | -47.5 | NC_003345.1 | + | 66431 | 0.66 | 0.996868 |
Target: 5'- ---uACGGCGcGAUgCGGgacagGAGUggcGCGGa -3' miRNA: 3'- cuuuUGCCGC-CUAaGCCa----UUCA---UGCC- -5' |
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12581 | 3' | -47.5 | NC_003345.1 | + | 67259 | 0.67 | 0.99393 |
Target: 5'- --cGACGGUGGGagUGGUGGGaACGa -3' miRNA: 3'- cuuUUGCCGCCUaaGCCAUUCaUGCc -5' |
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12581 | 3' | -47.5 | NC_003345.1 | + | 75706 | 0.72 | 0.914247 |
Target: 5'- ---uGCGGCGGAggcuguucUCGGUGGGggagaaGCGGc -3' miRNA: 3'- cuuuUGCCGCCUa-------AGCCAUUCa-----UGCC- -5' |
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12581 | 3' | -47.5 | NC_003345.1 | + | 77575 | 0.77 | 0.67113 |
Target: 5'- uGAAAACGGCGGAUUCGGU-------- -3' miRNA: 3'- -CUUUUGCCGCCUAAGCCAuucaugcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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