Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12605 | 3' | -57.6 | NC_003345.1 | + | 45346 | 0.66 | 0.696657 |
Target: 5'- ---cCGAGCUGAAccGCCGCCuucuCGCUc -3' miRNA: 3'- gcaaGCUCGACUU--CGGCGGccu-GCGG- -5' |
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12605 | 3' | -57.6 | NC_003345.1 | + | 62935 | 0.66 | 0.696657 |
Target: 5'- ----gGAGCUGGAGUaguuCGCCGGGucgagGCCg -3' miRNA: 3'- gcaagCUCGACUUCG----GCGGCCUg----CGG- -5' |
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12605 | 3' | -57.6 | NC_003345.1 | + | 28434 | 0.66 | 0.717163 |
Target: 5'- aCGUaugcCGAGUUGAuggGGCCGUgggaggauUGGGCGCa -3' miRNA: 3'- -GCAa---GCUCGACU---UCGGCG--------GCCUGCGg -5' |
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12605 | 3' | -57.6 | NC_003345.1 | + | 72637 | 0.66 | 0.717163 |
Target: 5'- --aUCGAGCagacggagGAAGauauugCGCCGGuCGCCc -3' miRNA: 3'- gcaAGCUCGa-------CUUCg-----GCGGCCuGCGG- -5' |
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12605 | 3' | -57.6 | NC_003345.1 | + | 50432 | 0.66 | 0.727306 |
Target: 5'- uCGUaUCGGGaggUGGAGCCGCUGG-CGg- -3' miRNA: 3'- -GCA-AGCUCg--ACUUCGGCGGCCuGCgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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