Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1261 | 3' | -51.3 | NC_001317.1 | + | 9159 | 0.66 | 0.745523 |
Target: 5'- cGGUUGCUAUgUaugcaaacGGUgaauuaGUGAGCCAGUg -3' miRNA: 3'- -CCAGCGAUAgAga------CCG------UAUUCGGUCG- -5' |
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1261 | 3' | -51.3 | NC_001317.1 | + | 7755 | 0.66 | 0.778555 |
Target: 5'- -aUCGCUgcGUUUCUGGaCAU--GCUGGCg -3' miRNA: 3'- ccAGCGA--UAGAGACC-GUAuuCGGUCG- -5' |
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1261 | 3' | -51.3 | NC_001317.1 | + | 5091 | 0.66 | 0.734226 |
Target: 5'- cGGUCGUg----CUGGCgAUGGuGCCGGUc -3' miRNA: 3'- -CCAGCGauagaGACCG-UAUU-CGGUCG- -5' |
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1261 | 3' | -51.3 | NC_001317.1 | + | 11707 | 0.66 | 0.767702 |
Target: 5'- --gCGCaggCUCUGGCAgcAGCCAc- -3' miRNA: 3'- ccaGCGauaGAGACCGUauUCGGUcg -5' |
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1261 | 3' | -51.3 | NC_001317.1 | + | 570 | 0.68 | 0.664441 |
Target: 5'- cGGUCGCg--CUCUGcGCcg-GGUCGGUc -3' miRNA: 3'- -CCAGCGauaGAGAC-CGuauUCGGUCG- -5' |
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1261 | 3' | -51.3 | NC_001317.1 | + | 21032 | 0.68 | 0.664441 |
Target: 5'- -aUCGCUugGUCgCUGGUucAAGuCCAGCa -3' miRNA: 3'- ccAGCGA--UAGaGACCGuaUUC-GGUCG- -5' |
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1261 | 3' | -51.3 | NC_001317.1 | + | 1090 | 0.68 | 0.664441 |
Target: 5'- cGGUCaGCUucUCcgggCUGGCAaaGAGCCuGCg -3' miRNA: 3'- -CCAG-CGAu-AGa---GACCGUa-UUCGGuCG- -5' |
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1261 | 3' | -51.3 | NC_001317.1 | + | 14233 | 0.7 | 0.501018 |
Target: 5'- cGGUCGCaaccgcaAUCgUC-GGCAUGgucuggacAGCCAGCg -3' miRNA: 3'- -CCAGCGa------UAG-AGaCCGUAU--------UCGGUCG- -5' |
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1261 | 3' | -51.3 | NC_001317.1 | + | 15687 | 0.73 | 0.357625 |
Target: 5'- -uUCGCcagUGUCgucacgCUGGUAaGAGCCAGCa -3' miRNA: 3'- ccAGCG---AUAGa-----GACCGUaUUCGGUCG- -5' |
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1261 | 3' | -51.3 | NC_001317.1 | + | 7917 | 0.77 | 0.206611 |
Target: 5'- uGGUCGCUguaaucggugaaaAUCUCUGGUcugccAUcaAGGCCAGUg -3' miRNA: 3'- -CCAGCGA-------------UAGAGACCG-----UA--UUCGGUCG- -5' |
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1261 | 3' | -51.3 | NC_001317.1 | + | 11005 | 1.16 | 0.000367 |
Target: 5'- aGGUCGCUAUCUCUGGCAUAAGCCAGCa -3' miRNA: 3'- -CCAGCGAUAGAGACCGUAUUCGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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