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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 1261 | 5' | -48.9 | NC_001317.1 | + | 26601 | 0.66 | 0.868533 |
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Target: 5'- uGGGaUAGCUUCAUaucgugacugggcgaUGACuACGCgUGGCGg -3' miRNA: 3'- gCUC-GUCGAAGUA---------------AUUG-UGCG-ACUGC- -5' |
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| 1261 | 5' | -48.9 | NC_001317.1 | + | 847 | 0.69 | 0.729664 |
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Target: 5'- -cAGUAGCUUgAUUAGCuggccgguaACGCUGugGc -3' miRNA: 3'- gcUCGUCGAAgUAAUUG---------UGCGACugC- -5' |
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| 1261 | 5' | -48.9 | NC_001317.1 | + | 18888 | 0.69 | 0.718023 |
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Target: 5'- -cAGUcauGGC-UCAUacUGGCACGCUGACGc -3' miRNA: 3'- gcUCG---UCGaAGUA--AUUGUGCGACUGC- -5' |
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| 1261 | 5' | -48.9 | NC_001317.1 | + | 28319 | 0.69 | 0.752579 |
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Target: 5'- uGAGCAGCaUCAU--GCACuGC-GGCGa -3' miRNA: 3'- gCUCGUCGaAGUAauUGUG-CGaCUGC- -5' |
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| 1261 | 5' | -48.9 | NC_001317.1 | + | 11923 | 0.72 | 0.562599 |
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Target: 5'- cCGAGCGGC-UCAaUAACuCGcCUGACa -3' miRNA: 3'- -GCUCGUCGaAGUaAUUGuGC-GACUGc -5' |
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| 1261 | 5' | -48.9 | NC_001317.1 | + | 10970 | 1.09 | 0.002058 |
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Target: 5'- gCGAGCAGCUUCAUUAACACGCUGACGc -3' miRNA: 3'- -GCUCGUCGAAGUAAUUGUGCGACUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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