miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1261 5' -48.9 NC_001317.1 + 847 0.69 0.729664
Target:  5'- -cAGUAGCUUgAUUAGCuggccgguaACGCUGugGc -3'
miRNA:   3'- gcUCGUCGAAgUAAUUG---------UGCGACugC- -5'
1261 5' -48.9 NC_001317.1 + 10970 1.09 0.002058
Target:  5'- gCGAGCAGCUUCAUUAACACGCUGACGc -3'
miRNA:   3'- -GCUCGUCGAAGUAAUUGUGCGACUGC- -5'
1261 5' -48.9 NC_001317.1 + 11923 0.72 0.562599
Target:  5'- cCGAGCGGC-UCAaUAACuCGcCUGACa -3'
miRNA:   3'- -GCUCGUCGaAGUaAUUGuGC-GACUGc -5'
1261 5' -48.9 NC_001317.1 + 18888 0.69 0.718023
Target:  5'- -cAGUcauGGC-UCAUacUGGCACGCUGACGc -3'
miRNA:   3'- gcUCG---UCGaAGUA--AUUGUGCGACUGC- -5'
1261 5' -48.9 NC_001317.1 + 26601 0.66 0.868533
Target:  5'- uGGGaUAGCUUCAUaucgugacugggcgaUGACuACGCgUGGCGg -3'
miRNA:   3'- gCUC-GUCGAAGUA---------------AUUG-UGCG-ACUGC- -5'
1261 5' -48.9 NC_001317.1 + 28319 0.69 0.752579
Target:  5'- uGAGCAGCaUCAU--GCACuGC-GGCGa -3'
miRNA:   3'- gCUCGUCGaAGUAauUGUG-CGaCUGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.