Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1264 | 3' | -55.9 | NC_001317.1 | + | 15529 | 1.09 | 0.000366 |
Target: 5'- aGCGCCAUUCAUACCGCCGCCGCGAUAg -3' miRNA: 3'- -CGCGGUAAGUAUGGCGGCGGCGCUAU- -5' |
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1264 | 3' | -55.9 | NC_001317.1 | + | 25357 | 0.73 | 0.167103 |
Target: 5'- uGUGCCAUUUAUaACCuuuaaaucgcccugGCuCGCCGCGAUAc -3' miRNA: 3'- -CGCGGUAAGUA-UGG--------------CG-GCGGCGCUAU- -5' |
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1264 | 3' | -55.9 | NC_001317.1 | + | 17810 | 0.73 | 0.183491 |
Target: 5'- gGCGgcuuCCAUcaGUACCGCCGCCGcCGAg- -3' miRNA: 3'- -CGC----GGUAagUAUGGCGGCGGC-GCUau -5' |
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1264 | 3' | -55.9 | NC_001317.1 | + | 2492 | 0.72 | 0.205246 |
Target: 5'- aGCGCCGggugcgUCGUGCucgCGCCGCCgGUGGUu -3' miRNA: 3'- -CGCGGUa-----AGUAUG---GCGGCGG-CGCUAu -5' |
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1264 | 3' | -55.9 | NC_001317.1 | + | 2302 | 0.71 | 0.24204 |
Target: 5'- uGCGCuCAUUC-UACCcgGCCGCaCGCGGc- -3' miRNA: 3'- -CGCG-GUAAGuAUGG--CGGCG-GCGCUau -5' |
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1264 | 3' | -55.9 | NC_001317.1 | + | 3441 | 0.69 | 0.340382 |
Target: 5'- cGUGCCAgUCGUucaauCUGCCGCC-CGAg- -3' miRNA: 3'- -CGCGGUaAGUAu----GGCGGCGGcGCUau -5' |
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1264 | 3' | -55.9 | NC_001317.1 | + | 15592 | 0.69 | 0.340382 |
Target: 5'- cGCGCCGggggcUgAUGCCguGCCGCUGCGu-- -3' miRNA: 3'- -CGCGGUa----AgUAUGG--CGGCGGCGCuau -5' |
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1264 | 3' | -55.9 | NC_001317.1 | + | 20605 | 0.69 | 0.343826 |
Target: 5'- aGUGCCA-UCAUGCCGCacaugcgcgaacaaGCCGCu--- -3' miRNA: 3'- -CGCGGUaAGUAUGGCGg-------------CGGCGcuau -5' |
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1264 | 3' | -55.9 | NC_001317.1 | + | 17545 | 0.69 | 0.349041 |
Target: 5'- cGCGCCAUUCucaGCCGCCuGCaGCu--- -3' miRNA: 3'- -CGCGGUAAGua-UGGCGG-CGgCGcuau -5' |
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1264 | 3' | -55.9 | NC_001317.1 | + | 6969 | 0.67 | 0.443942 |
Target: 5'- uGCGCCAg----ACUGCCGCCGg---- -3' miRNA: 3'- -CGCGGUaaguaUGGCGGCGGCgcuau -5' |
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1264 | 3' | -55.9 | NC_001317.1 | + | 24269 | 0.67 | 0.413943 |
Target: 5'- aCGCgAagUAUugCGCCGUCGCGGg- -3' miRNA: 3'- cGCGgUaaGUAugGCGGCGGCGCUau -5' |
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1264 | 3' | -55.9 | NC_001317.1 | + | 26854 | 0.66 | 0.464606 |
Target: 5'- -aGCCGcgaaCAUACgGUCGCgGCGAUGc -3' miRNA: 3'- cgCGGUaa--GUAUGgCGGCGgCGCUAU- -5' |
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1264 | 3' | -55.9 | NC_001317.1 | + | 3564 | 0.66 | 0.48576 |
Target: 5'- cGCGCCugAUUCAgcucAUCGCCaaGCCGCa--- -3' miRNA: 3'- -CGCGG--UAAGUa---UGGCGG--CGGCGcuau -5' |
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1264 | 3' | -55.9 | NC_001317.1 | + | 9541 | 0.66 | 0.517215 |
Target: 5'- cGCGCCAUgcgcugCAccuUGCCGguaaacuccaccaCCGCCGCa--- -3' miRNA: 3'- -CGCGGUAa-----GU---AUGGC-------------GGCGGCGcuau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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