miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1264 5' -54.1 NC_001317.1 + 3625 0.66 0.588483
Target:  5'- -gCAGCauUUCGGCAaucuGCGgCACGGAa-- -3'
miRNA:   3'- caGUCG--AAGCCGU----CGCaGUGCCUaag -5'
1264 5' -54.1 NC_001317.1 + 19759 0.69 0.380882
Target:  5'- --aAGCgcaauaaacugaCGGcCAGUGUCGCGGAUUCa -3'
miRNA:   3'- cagUCGaa----------GCC-GUCGCAGUGCCUAAG- -5'
1264 5' -54.1 NC_001317.1 + 639 0.7 0.3569
Target:  5'- ---uGCggCGGaaCAGCGUUGCGGAUUCa -3'
miRNA:   3'- caguCGaaGCC--GUCGCAGUGCCUAAG- -5'
1264 5' -54.1 NC_001317.1 + 1772 0.7 0.3569
Target:  5'- uGUCAGCgcaUCGaGCAGCGccUCACGGc--- -3'
miRNA:   3'- -CAGUCGa--AGC-CGUCGC--AGUGCCuaag -5'
1264 5' -54.1 NC_001317.1 + 8463 0.71 0.313829
Target:  5'- --gAGCUguUCGcGCAGUGUCaccugcGCGGAUUCa -3'
miRNA:   3'- cagUCGA--AGC-CGUCGCAG------UGCCUAAG- -5'
1264 5' -54.1 NC_001317.1 + 15487 1.1 0.000448
Target:  5'- gGUCAGCUUCGGCAGCGUCACGGAUUCa -3'
miRNA:   3'- -CAGUCGAAGCCGUCGCAGUGCCUAAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.